α-羟基羧酸盐和二醇对映选择性指示剂置换测定法的实施指南。
Guidelines in implementing enantioselective indicator-displacement assays for alpha-hydroxycarboxylates and diols.
作者信息
Zhu Lei, Zhong Zhenlin, Anslyn Eric V
机构信息
Department of Chemistry and Biochemistry, the University of Texas at Austin, Austin, Texas 78712, USA.
出版信息
J Am Chem Soc. 2005 Mar 30;127(12):4260-9. doi: 10.1021/ja0435945.
Enantioselective association events between boronic acid receptors and bifunctional substrates such as alpha-hydroxycarboxylates and vicinal diols were exploited to develop enantioselective indicator-displacement assays. It was found that the sensitivity of the assay could be tuned by using either colorimetric or fluorescent indicators. Mathematical principles were established for concurrent determination of concentration and enantiomeric excess (ee) of a chiral analyte, which has been a challenge to the high-throughput analysis of chiral samples. Furthermore, experimental protocols were optimized to maximize the chiral resolution of the assay and to improve the accuracy of the ee measurements.
利用硼酸受体与双功能底物（如α-羟基羧酸盐和邻二醇）之间的对映选择性缔合事件，开发了对映选择性指示剂置换分析方法。研究发现，使用比色或荧光指示剂均可调节分析的灵敏度。建立了用于同时测定手性分析物浓度和对映体过量（ee）的数学原理，这一直是手性样品高通量分析面临的挑战。此外，还优化了实验方案，以最大化分析的手性分辨率并提高ee测量的准确性。
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