通过指示剂置换分析法轻松定量对映体过量和浓度：α-羟基酸分析实例
Facile quantification of enantiomeric excess and concentration with indicator-displacement assays: an example in the analyses of alpha-hydroxyacids.
作者信息
Zhu Lei, Anslyn Eric V
机构信息
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA.
出版信息
J Am Chem Soc. 2004 Mar 31;126(12):3676-7. doi: 10.1021/ja031839s.
PMID:15038696
Abstract
An enantioselective indicator-displacement assay was developed to determine enantiomeric excess (ee) and concentration of alpha-hydroxyacid samples with two independent absorption measurements. The inherent relationship between the absorbance of the indicator-displacement ensemble and the overall concentration and ee of the analyte was established through solution equilibria.
摘要
开发了一种对映选择性指示剂置换分析法，通过两次独立的吸光度测量来测定α-羟基酸样品的对映体过量（ee）和浓度。通过溶液平衡建立了指示剂置换体系的吸光度与分析物的总浓度和ee之间的内在关系。
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