CD95及可溶性CD95表达与肝移植急性排斥反应状态的相关性
Correlation of CD95 and soluble CD95 expression with acute rejection status of liver transplantation.
作者信息
Wang Yu-Liang, Zhang Yan-Yan, Li Guang, Tang Zhi-Qin, Zhou Yan-Li, Zhu Zhi-Jun, Yao Zhi
机构信息
Tianjin Institute of Thrombosis and Hemostasis, Laboratory Center, Tianjin First Central Hospital, 24 FuKang Road, Tianjin 300192, China.
出版信息
World J Gastroenterol. 2005 Mar 21;11(11):1700-4. doi: 10.3748/wjg.v11.i11.1700.
AIM
To analyze the expression levels of soluble form of CD95, CD95 ligand (sCD95 and sCD95L, respectively) in plasma and CD95 expression on CD3+ cells in liver-transplanted recipients with acute rejection (AR).
METHODS
Peripheral blood mononuclear cells (PBMCs) were isolated from 30 clinically liver transplanted recipients. CD95 expression on CD3+ cells was quantitatively measured by two-color fluorescence activated cell sorter (FACS) analysis. Lymphocyte surface phenotypes of CD4, CD8, CD16 and CD56 were determined by flow cytometry. Plasma levels of sCD95 and sCD95L were detected by Enzyme Linked-Immuno-Sorbent Assay (ELISA). The results were compared with that from normal healthy volunteers (n = 15 individuals).
RESULTS
FACS analysis showed that CD95 expression on CD3+ T cells was significantly increased in liver transplanted recipients with AR compared to that in stable recipients without rejection and infection or healthy individuals who did not undergo transplantation (18,676.93+/-11,588.34/molecule, 6,848.20+/-1 712.96/molecule, 6,418.01+/-2,001.95/molecule, respectively, P<0.01). Whereas no significant difference was seen between liver-transplanted stable recipients and healthy individuals. Furthermore, no significant differences were detected between each group with CD4/CD8 ratio or the percentage of CD16+56+ cells. Plasma levels of sCD95 were significantly higher in transplanted recipients with AR compared to that in stable recipients or healthy individuals (391.88+/-196.00, 201.37+/-30.30, 148.83+/-58.25 pg/mL, respectively, P<0.01). In contrast, the plasma levels of sCD95L in liver- transplanted recipients were not significantly different from that in healthy individuals.
CONCLUSION
The present results indicate that the increased CD95 expression on CD3+ cells and the increased levels of sCD95 in plasma may modify the immunological situation of the recipients after transplantation or represent the ongoing graft rejection.
目的
分析急性排斥反应（AR）肝移植受者血浆中可溶性形式的CD95、CD95配体（分别为sCD95和sCD95L）的表达水平以及CD3⁺细胞上CD95的表达情况。
方法
从30例临床肝移植受者中分离外周血单个核细胞（PBMC）。通过双色荧光激活细胞分选仪（FACS）分析定量检测CD3⁺细胞上CD95的表达。采用流式细胞术测定CD4、CD8、CD16和CD56的淋巴细胞表面表型。采用酶联免疫吸附测定法（ELISA）检测血浆中sCD95和sCD95L的水平。将结果与正常健康志愿者（n = 15人）的结果进行比较。
结果
FACS分析显示，与无排斥反应和感染的稳定受者或未接受移植的健康个体相比，发生AR的肝移植受者CD3⁺T细胞上CD95的表达显著增加（分别为18,676.93±11,588.34/分子、6,848.20±1712.96/分子、6,418.01±2,001.95/分子，P<0.01）。而肝移植稳定受者与健康个体之间未见显著差异。此外，各组之间在CD4/CD8比值或CD16⁺56⁺细胞百分比方面未检测到显著差异。与稳定受者或健康个体相比，发生AR的移植受者血浆中sCD95水平显著更高（分别为391.88±196.00、201.37±30.30、148.83±58.25 pg/mL，P<0.01）。相比之下，肝移植受者血浆中sCD95L水平与健康个体无显著差异。
结论
目前结果表明，CD3⁺细胞上CD95表达增加以及血浆中sCD95水平升高可能改变移植后受者的免疫状况或代表正在发生的移植物排斥反应。
Zotero 插件