埃及伊蚊卵壳黑化过程中一种前酚氧化酶的鉴定及分子特征分析
Identification and molecular characterization of a prophenoloxidase involved in Aedes aegypti chorion melanization.
作者信息
Kim S R, Yao R, Han Q, Christensen B M, Li J
机构信息
Department of Pathobiology, University of Illinois at Urbana-Champain, Urbana, IL 61802, USA.
出版信息
Insect Mol Biol. 2005 Apr;14(2):185-94. doi: 10.1111/j.1365-2583.2004.00547.x.
Abstract
Chorion melanization is a vital biochemical event for the survival of mosquito eggs in the environment. This study describes the identification and molecular characterization of a prophenoloxidase (proPO) involved in chorion melanization in Aedes aegypti by various biochemical and molecular techniques. Results revealed that transcription of the chorion proPO occurs only in adults, blood feeding greatly stimulated its transcription and haemocytes are responsible for its transcription. Our study provides a solid basis for suggesting an essential role of the isolated proPO in chorion melanization during chorion hardening and also raises fundamental questions regarding its transportation and distribution in the chorion. This study should serve as a useful reference towards functional elucidation of other proPOs in mosquitoes.
摘要
卵壳黑化是蚊卵在环境中存活的关键生化事件。本研究通过各种生化和分子技术描述了埃及伊蚊中参与卵壳黑化的一种前酚氧化酶（proPO）的鉴定及其分子特征。结果显示，卵壳proPO的转录仅在成虫中发生，血液喂养极大地刺激了其转录，并且血细胞负责其转录。我们的研究为所分离的proPO在卵壳硬化过程中的卵壳黑化中发挥重要作用提供了坚实基础，同时也提出了关于其在卵壳中的运输和分布的基本问题。本研究应为阐明蚊子中其他proPO的功能提供有用参考。
相似文献
1
Identification and molecular characterization of a prophenoloxidase involved in Aedes aegypti chorion melanization.埃及伊蚊卵壳黑化过程中一种前酚氧化酶的鉴定及分子特征分析
Insect Mol Biol. 2005 Apr;14(2):185-94. doi: 10.1111/j.1365-2583.2004.00547.x.
2
The dual roles of Armigeres subalbatus prophenoloxidase V in parasite melanization and egg chorion melanization in the mosquito Ar. subalbatus.白纹伊蚊前酚氧化酶V在该蚊种寄生虫黑化和卵壳黑化中的双重作用
Insect Biochem Mol Biol. 2015 Sep;64:68-77. doi: 10.1016/j.ibmb.2015.07.016. Epub 2015 Jul 27.
3
Purification and primary structural characterization of prophenoloxidases from Aedes aegypti larvae.埃及伊蚊幼虫酚氧化酶原的纯化及一级结构表征
Insect Biochem Mol Biol. 2005 Nov;35(11):1269-83. doi: 10.1016/j.ibmb.2005.07.001. Epub 2005 Aug 9.
4
Molecular cloning of two prophenoloxidase genes from the mosquito Aedes aegypti.从埃及伊蚊中克隆两个前酚氧化酶基因的分子克隆
Insect Mol Biol. 2001 Feb;10(1):97-103. doi: 10.1046/j.1365-2583.2001.00242.x.
5
Molecular cloning and characterisation of prophenoloxidase from haemocytes of the white shrimp, Litopenaeus vannamei.凡纳滨对虾血细胞中酚氧化酶原的分子克隆与特性分析
Fish Shellfish Immunol. 2005 May;18(5):417-30. doi: 10.1016/j.fsi.2004.10.004. Epub 2005 Jan 25.
6
A novel type of prophenoloxidase from the kuruma prawn Marsupenaeus japonicus contributes to the melanization of plasma in crustaceans.一种新型的来自日本对虾的酚氧化酶原有助于甲壳动物的血浆黑化。
Fish Shellfish Immunol. 2012 Jan;32(1):61-8. doi: 10.1016/j.fsi.2011.10.020. Epub 2011 Oct 29.
7
Molecular characterization of a prophenoloxidase cDNA from the malaria mosquito Anopheles stephensi.来自疟疾蚊子斯氏按蚊的一种前酚氧化酶cDNA的分子特征分析。
Insect Mol Biol. 2000 Apr;9(2):127-37. doi: 10.1046/j.1365-2583.2000.00169.x.
8
Prophenoloxidase from Pieris rapae: gene cloning, activity, and transcription in response to venom/calyx fluid from the endoparasitoid wasp Cotesia glomerata.甘蓝夜蛾酚氧化酶原：基因克隆、活性及其对鳞翅目内寄生蜂金小蜂毒液/花萼液的转录响应。
J Zhejiang Univ Sci B. 2011 Feb;12(2):103-15. doi: 10.1631/jzus.B1000275.
9
Cloning the prophenoloxidase cDNA and monitoring the expression of proPO mRNA in prawns (Macrobrachium rosenbergii) stimulated in vivo by CpG oligodeoxynucleotides.克隆罗氏沼虾酚氧化酶原cDNA并监测经CpG寡脱氧核苷酸体内刺激的罗氏沼虾中酚氧化酶原mRNA的表达。
Fish Shellfish Immunol. 2006 Mar;20(3):274-84. doi: 10.1016/j.fsi.2005.05.001. Epub 2005 Jun 14.
10
Activation of Aedes aegypti prophenoloxidase-3 and its role in the immune response against entomopathogenic fungi.埃及伊蚊前酚氧化酶-3的激活及其在抗昆虫病原真菌免疫反应中的作用。
Insect Mol Biol. 2017 Oct;26(5):552-563. doi: 10.1111/imb.12318. Epub 2017 May 27.
引用本文的文献
1
Precise coordination between nutrient transporters ensures fertility in the malaria mosquito Anopheles gambiae.精确协调营养转运蛋白可确保疟蚊冈比亚按蚊的生育能力。
PLoS Genet. 2024 Jan 29;20(1):e1011145. doi: 10.1371/journal.pgen.1011145. eCollection 2024 Jan.
2
Characterization of essential eggshell proteins from Aedes aegypti mosquitoes.从埃及伊蚊中鉴定必需的卵壳蛋白。
BMC Biol. 2023 Oct 13;21(1):214. doi: 10.1186/s12915-023-01721-z.
3
Metal ions in insect reproduction: a crosstalk between reproductive physiology and immunity.昆虫生殖中的金属离子：生殖生理学与免疫之间的串扰。
Curr Opin Insect Sci. 2022 Aug;52:100924. doi: 10.1016/j.cois.2022.100924. Epub 2022 Apr 25.
4
Aquatain® causes anti-oviposition, egg retention and oocyte melanization and triggers female death in Aedes aegypti.Aquatain® 导致埃及伊蚊抗产卵、卵滞留和卵母细胞黑化，并引发雌性死亡。
Parasit Vectors. 2022 Mar 22;15(1):100. doi: 10.1186/s13071-022-05202-0.
5
Yellow-y Functions in Egg Melanization and Chorion Morphology of the Asian Tiger Mosquito, .黄色-y基因在亚洲虎蚊卵黑化和卵壳形态中的作用
Front Cell Dev Biol. 2021 Dec 16;9:769788. doi: 10.3389/fcell.2021.769788. eCollection 2021.
6
Transcriptome profiling reveals the developmental regulation of NaCl-treated Forcipomyia taiwana eggs.转录组谱分析揭示了 NaCl 处理台湾蚋卵的发育调控。
BMC Genomics. 2021 Nov 3;22(1):792. doi: 10.1186/s12864-021-08096-x.
7
Immunity for nothing and the eggs for free: Apparent lack of both physiological trade-offs and terminal reproductive investment in female crickets (Gryllus texensis).不劳而获：雌性蟋蟀（Gryllus texensis）似乎不存在生理权衡和终极生殖投资。
PLoS One. 2019 May 15;14(5):e0209957. doi: 10.1371/journal.pone.0209957. eCollection 2019.
8
Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes.鉴定和描述埃及伊蚊中一种蚊子特异性卵壳形成因子。
PLoS Biol. 2019 Jan 8;17(1):e3000068. doi: 10.1371/journal.pbio.3000068. eCollection 2019 Jan.
9
Mosquito Innate Immunity.蚊子的先天免疫
Insects. 2018 Aug 8;9(3):95. doi: 10.3390/insects9030095.
10
Coffee and its waste repel gravid Aedes albopictus females and inhibit the development of their embryos.咖啡及其废弃物可驱赶妊娠白纹伊蚊雌蚊，并抑制其胚胎发育。
Parasit Vectors. 2015 May 14;8:272. doi: 10.1186/s13071-015-0874-6.
本文引用的文献
1
Comparative and functional genomics of the innate immune system in the malaria vector Anopheles gambiae.冈比亚按蚊（疟疾传播媒介）先天免疫系统的比较与功能基因组学
Immunol Rev. 2004 Apr;198:127-48. doi: 10.1111/j.0105-2896.2004.0127.x.
2
The prophenoloxidase-activating system in invertebrates.无脊椎动物中的酚氧化酶原激活系统。
Immunol Rev. 2004 Apr;198:116-26. doi: 10.1111/j.0105-2896.2004.00116.x.
3
Innate immune responses of a lepidopteran insect, Manduca sexta.一种鳞翅目昆虫烟草天蛾的先天免疫反应。
Immunol Rev. 2004 Apr;198:97-105. doi: 10.1111/j.0105-2896.2004.0121.x.
4
Prophenoloxidase-activating proteinase-3 (PAP-3) from Manduca sexta hemolymph: a clip-domain serine proteinase regulated by serpin-1J and serine proteinase homologs.烟草天蛾血淋巴中的酚氧化酶原激活蛋白酶-3（PAP-3）：一种受丝氨酸蛋白酶抑制剂-1J和丝氨酸蛋白酶同源物调控的clip结构域丝氨酸蛋白酶
Insect Biochem Mol Biol. 2003 Oct;33(10):1049-60. doi: 10.1016/s0965-1748(03)00123-1.
5
Hemocyte-mediated phagocytosis and melanization in the mosquito Armigeres subalbatus following immune challenge by bacteria.细菌免疫攻击后，白纹伊蚊血细胞介导的吞噬作用和黑化作用
Cell Tissue Res. 2003 Jul;313(1):117-27. doi: 10.1007/s00441-003-0744-y. Epub 2003 Jun 28.
6
Prophenol oxidase A3 in Drosophila melanogaster: activation and the PCR-based cDNA sequence.黑腹果蝇中的酚氧化酶原A3：激活与基于PCR的cDNA序列
Biochem Genet. 2003 Jun;41(5-6):151-63. doi: 10.1023/a:1023325610300.
7
Comparative biochemistry of eumelanogenesis and the protective roles of phenoloxidase and melanin in insects.昆虫中真黑素生成的比较生物化学以及酚氧化酶和黑色素的保护作用
Pigment Cell Res. 2002 Feb;15(1):2-9. doi: 10.1034/j.1600-0749.2002.00056.x.
8
Effect of prophenoloxidase expression knockout on the melanization of microfilariae in the mosquito Armigeres subalbatus.酚氧化酶原表达敲除对骚扰阿蚊体内微丝蚴黑化的影响
Insect Mol Biol. 2001 Aug;10(4):315-21. doi: 10.1046/j.0962-1075.2001.00268.x.
9
Molecular cloning of two prophenoloxidase genes from the mosquito Aedes aegypti.从埃及伊蚊中克隆两个前酚氧化酶基因的分子克隆
Insect Mol Biol. 2001 Feb;10(1):97-103. doi: 10.1046/j.1365-2583.2001.00242.x.
10
Molecular cloning of a second prophenoloxidase cDNA from the mosquito Armigeres subalbatus: prophenoloxidase expression in blood-fed and microfilariae-inoculated mosquitoes.致倦库蚊第二个酚氧化酶原cDNA的分子克隆：酚氧化酶原在吸血和接种微丝蚴蚊虫中的表达
Insect Mol Biol. 2001 Feb;10(1):87-96. doi: 10.1046/j.1365-2583.2001.00241.x.
Zotero 插件