University of Tennessee, Department of Comparative Medicine, College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Jan 15;879(2):205-8. doi: 10.1016/j.jchromb.2010.11.026. Epub 2010 Dec 3.
A new method of analysis has been developed and validated for the determination of firocoxib, a new nonsteroidal anti-inflammatory drug (NSAID) approved for use in horses and dogs to control pain and inflammation associated with osteoarthritis. Following a liquid extraction using ethyl acetate:hexane (40:60), samples were separated by isocratic reversed-phase HPLC on a Sunfire C(18) column and quantified using UV detection at 290 nm. The mobile phase was a mixture of water with 0.025% trifluoroacetic acid and acetonitrile, with a flow-rate of 1.1 ml/min. The procedure produced a linear curve over the concentration range 5-1500 ng/ml with a lower limit of quantification of 5 ng/ml. Intra- and inter-assay variability was less than 7%. The average recovery was 98%. The method is suitable for the analysis of clinical samples from pharmacokinetic studies and can also be used for small volume sample sizes.
已开发并验证了一种新的分析方法，用于测定非甾体抗炎药（NSAID）昔布考昔的含量。昔布考昔是一种新型药物，已获准用于马和犬，用于控制与骨关节炎相关的疼痛和炎症。样品经乙酸乙酯：正己烷（40:60）液液萃取后，采用 Sunfire C(18)柱进行等度反相高效液相色谱分离，并在 290nm 处进行紫外检测定量。流动相为含 0.025%三氟乙酸的水和乙腈的混合物，流速为 1.1ml/min。该方法在 5-1500ng/ml 的浓度范围内呈线性曲线，定量下限为 5ng/ml。日内和日间变异性均小于 7%。平均回收率为 98%。该方法适用于药代动力学研究中临床样品的分析，也可用于小体积样品的分析。
