Andreassi Maria Grazia, Cioppa Angelo, Botto Nicoletta, Joksic Gordana, Manfredi Samantha, Federici Chiara, Ostojic Miodrag, Rubino Paolo, Picano Eugenio
Institute of Clinical Physiology CNR, Pisa, Italy.
FASEB J. 2005 Jun;19(8):998-9. doi: 10.1096/fj.04-3287fje. Epub 2005 Mar 31.
Interventional cardiologists who work in cardiac catheterization laboratories are exposed to low doses of ionizing radiation that could pose a health hazard. DNA damage is considered to be the main initiating event by which radiation damage to cells results in development of cancer and hereditary disease. The aim of the present study was to assess the effects of chronic low-dose X-ray radiation exposure on somatic DNA damage of interventional cardiologists working in high-volume cardiac catheterization laboratories. For this analysis, we used peripheral lymphocytes and the assay for micronuclei (MNs), which is considered to be a reliable biological dosimeter for radiation exposure. We obtained peripheral blood from 62 physicians (mean age+/-se = 40.6+/-1.5 years): 31 interventional cardiologists (group I, exposed) and 31 age- and sex-matched clinical cardiologists (group II, nonexposed). Interventional cardiologists showed higher MN values (group I=20.5+/-1.6 vs. group II=12.8+/-1.3, P=0.001), although some overlap was apparent in the individual subject analysis. A correlation between years of professional activity and MN frequency value was detectable for interventional cardiologists (r=0.428, P=0.02) but not for clinical cardiologists (r=0.253, P=0.17). The results indicated that, overall, interventional cardiologists working in a high-volume catheterization laboratory have higher levels of somatic DNA damage when compared with clinical cardiologists working outside the catheterization laboratory. The amount of this damage varies and is only weakly related to the duration of professional exposure, which suggests that a dominant modulation of the underlying genetic substrate by environmental factors has a role in determining the harm in individual physicians.
在心脏导管实验室工作的介入心脏病专家会接触到低剂量的电离辐射，这可能对健康构成危害。DNA损伤被认为是辐射对细胞造成损害进而导致癌症和遗传性疾病发生的主要起始事件。本研究的目的是评估长期低剂量X射线辐射暴露对在大量心脏导管实验室工作的介入心脏病专家体细胞DNA损伤的影响。为此分析，我们使用外周血淋巴细胞和微核（MN）检测法，该方法被认为是一种可靠的辐射暴露生物剂量计。我们从62名医生（平均年龄±标准误 = 40.6±1.5岁）获取外周血：31名介入心脏病专家（I组，暴露组）和31名年龄及性别匹配的临床心脏病专家（II组，非暴露组）。介入心脏病专家的MN值更高（I组 = 20.5±1.6 vs. II组 = 12.8±1.3，P = 0.001），尽管在个体受试者分析中存在一些重叠。介入心脏病专家的专业活动年限与MN频率值之间存在相关性（r = 0.428，P = 0.02），而临床心脏病专家则不存在这种相关性（r = 0.253，P = 0.17）。结果表明，总体而言，与在导管实验室外工作的临床心脏病专家相比，在大量导管实验室工作的介入心脏病专家体细胞DNA损伤水平更高。这种损伤的程度各不相同，且与职业暴露时间仅存在微弱关联，这表明环境因素对潜在遗传底物的主要调节作用在决定个体医生所受危害方面发挥了作用。
