固定化的N-烷基化聚乙烯亚胺通过破坏细胞膜来迅速杀死细菌，且不会产生耐药性。

Immobilized N-alkylated polyethylenimine avidly kills bacteria by rupturing cell membranes with no resistance developed.

作者信息

Milović Nebojsa M, Wang Jun, Lewis Kim, Klibanov Alexander M

机构信息

Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

出版信息

Biotechnol Bioeng. 2005 Jun 20;90(6):715-22. doi: 10.1002/bit.20454.

DOI:10.1002/bit.20454

PMID:15803464

Abstract

Several critical mechanistic and phenomenological aspects of the microbicidal surface coatings based on immobilized hydrophobic polycations, previously developed by us, are addressed. Using Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria, remarkable bactericidal action (up to a 10(9)-fold reduction in live bacteria count in the surface-exposed solution and a 100% inactivation of the surface-adhered bacteria) of an amino-glass slide covalently derivatized with N-hexyl,methyl-polyethylenimine (PEI) is found to be due to rupturing bacterial cell membranes by the polymeric chains. The bacteria fail to develop noticeable resistance to this lethal action over the course of many successive generations. Finally, the immobilized N-alkyl-PEI, while deadly to bacteria, is determined to be harmless to mammalian (monkey kidney) cells.

摘要

本文探讨了我们之前开发的基于固定化疏水聚阳离子的杀菌表面涂层的几个关键机理和现象学方面。使用大肠杆菌（革兰氏阴性）和金黄色葡萄球菌（革兰氏阳性）细菌，发现用N-己基、甲基-聚乙烯亚胺（PEI）共价衍生的氨基玻片具有显著的杀菌作用（表面暴露溶液中的活菌数最多可减少10^9倍，表面附着的细菌100%失活），这是由于聚合物链破坏了细菌细胞膜。在许多连续代次的过程中，细菌对这种致命作用未能产生明显的抗性。最后，确定固定化的N-烷基-PEI虽然对细菌致命，但对哺乳动物（猴肾）细胞无害。