Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Biomaterials. 2010 May;31(14):4079-87. doi: 10.1016/j.biomaterials.2010.01.119. Epub 2010 Feb 18.
In this work, we designed contact-killing ionically cross-linked polymeric thin films using Layer-by-Layer (LbL) technology. A polycation, N,N-dodecyl,methyl-polyethylenimine, with microbicidal activity was layered with a polyanion, such as poly(acrylic acid), to create LbL films highly effective against both airborne and waterborne Escherichia coli and Staphylococcus aureus (Gram negative and positive bacteria, respectively), as well as influenza A/WSN (H1N1) virus. The dependence of the microbicidal activity on the pH during and post-assembly of LbL film formation, the nature of the polycation and polyanion, the number of layers in the LbL film, and other experimental variables was investigated quantitatively.
在这项工作中，我们使用层层（LbL）技术设计了具有接触杀菌作用的离子交联聚合物薄膜。带杀菌活性的聚阳离子，如 N,N-十二烷基甲基聚亚乙基亚胺，与聚阴离子（如聚丙烯酸）层层组装，形成的 LbL 薄膜对空气传播和水传播的大肠杆菌和金黄色葡萄球菌（分别为革兰氏阴性和阳性细菌）以及流感 A/WSN（H1N1）病毒具有高效的杀灭作用。定量研究了 LbL 薄膜形成过程中和形成后 pH 值、聚阳离子和聚阴离子的性质、LbL 薄膜的层数以及其他实验变量对杀菌活性的影响。
