Zaia Rachel, Quinto Giovanna M, Camargo Livia C S, Ribeiro Rodrigo T, Carmona-Ribeiro Ana M
Biocolloids Laboratory, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Avenida Professor Lineu Prestes, 748, Butantan, São Paulo 05508-000, Brazil.
Pharmaceuticals (Basel). 2023 May 30;16(6):816. doi: 10.3390/ph16060816.
Cationic and hydrophilic coatings based on casting and drying water dispersions of two different nanoparticles (NPs) onto glass are here described and evaluated for antimicrobial activity. Discoid cationic bilayer fragments (BF) surrounded by carboxy-methylcellulose (CMC) and poly (diallyl dimethyl ammonium) chloride (PDDA) NPs and spherical gramicidin D (Gr) NPs dispersed in water solution were cast onto glass coverslips and dried, forming a coating quantitatively evaluated against , and . From plating and colony forming units (CFU) counting, all strains interacting for 1 h with the coatings lost viability from 10 to 10, to zero CFU, at two sets of Gr and PDDA doses: 4.6 and 25 μg, respectively, or, 0.94 and 5 μg, respectively. Combinations produced broad spectrum, antimicrobial coatings; PDDA electrostatically attached to the microbes damaging cell walls, allowing Gr NPs interaction with the cell membrane. This concerted action promoted optimal activity at low Gr and PDDA doses. Further washing and drying of the deposited dried coatings showed that they were washed out so that antimicrobial activity was no longer present on the glass surface. Significant applications in biomedical materials can be foreseen for these transient coatings.
本文描述并评估了基于将两种不同纳米颗粒（NPs）的水分散体浇铸并干燥在玻璃上而形成的阳离子和亲水涂层的抗菌活性。将由羧甲基纤维素（CMC）和聚二烯丙基二甲基氯化铵（PDDA）纳米颗粒包围的盘状阳离子双层片段（BF）以及分散在水溶液中的球形短杆菌肽D（Gr）纳米颗粒浇铸在玻璃盖玻片上并干燥，形成一种针对 、 和 进行定量评估的涂层。通过平板接种和菌落形成单位（CFU）计数，在两组Gr和PDDA剂量下，即分别为4.6和25μg，或分别为0.94和5μg时，所有与涂层相互作用1小时的菌株的活力从10到10 ，直至CFU为零。组合产生了广谱抗菌涂层；PDDA静电附着在微生物上破坏细胞壁，使Gr纳米颗粒能够与细胞膜相互作用。这种协同作用在低Gr和PDDA剂量下促进了最佳活性。对沉积的干燥涂层进行进一步洗涤和干燥表明，它们被洗去，因此玻璃表面不再具有抗菌活性。可以预见这些瞬态涂层在生物医学材料中的重要应用。
