Pfefferkorn E R, Pfefferkorn L C
J Parasitol. 1979 Jun;65(3):364-70.
The induction of mutants resistant to 5-fluorodeoxyuridine (FUDR) was used to measure the efficiency of various physical and chemical mutagens on extracellular and intracellular Toxoplasma gondii. The frequency of resistant mutant was measured by plaque assay in human fibroblast cultures in the presence and absence of FUDR. When considered as a function of lethality, the most efficient mutagenesis was obtained with nitrosoguanidine treatment of extracellular parasites and with ethylmethane sulfonate treatment of actively growing intracellular parasites. Each of these treatments increased the frequency of FUDR-resistant mutants from less than one to more than 200 per million parasites. Ultraviolet irradiation, X-rays, and the alkylating mustard ICR-191 also induced FUDR-resistant mutants in a dose-dependent fashion.
利用诱导对5-氟脱氧尿苷（FUDR）耐药的突变体来测定各种物理和化学诱变剂对细胞外和细胞内刚地弓形虫的诱变效率。通过在有和没有FUDR存在的情况下，在人成纤维细胞培养物中进行噬斑测定来测量耐药突变体的频率。当将诱变效率视为致死率的函数时，用亚硝基胍处理细胞外寄生虫以及用甲基磺酸乙酯处理活跃生长的细胞内寄生虫可获得最高效的诱变效果。这些处理中的每一种都使每百万寄生虫中FUDR耐药突变体的频率从少于1个增加到超过200个。紫外线照射、X射线以及烷基化芥子气ICR-191也以剂量依赖的方式诱导出FUDR耐药突变体。
