Pfefferkorn E R, Pfefferkorn L C
J Parasitol. 1976 Dec;62(6):993-9.
Adenine arabinoside, which inhibits the synthesis of DNA by intracellular Toxoplasma gondii, is more inhibitory to the parasite than it is to cultured human fibroblast host cells. A single-step mutant of T. gondii that is 50-fold more resistant to adenine arabinoside was isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Cytosine arabinoside is notably more inhibitory to cultured human cells than it is to T. gondii. Thus in infected cells treated with cytosine arabinoside and labeled with 3H-deoxyuridine nearly all of the label is specifically incorporated into the intracellular T. gondii.
阿糖腺苷可抑制细胞内刚地弓形虫的DNA合成，对该寄生虫的抑制作用比对培养的人成纤维细胞宿主细胞更强。在用N-甲基-N'-硝基-N-亚硝基胍诱变后，分离出了一株对阿糖腺苷的抗性比野生型高50倍的刚地弓形虫单步突变体。阿糖胞苷对培养的人细胞的抑制作用明显比对刚地弓形虫更强。因此，在用阿糖胞苷处理并用3H-脱氧尿苷标记的感染细胞中，几乎所有的标记都特异性地掺入到细胞内的刚地弓形虫中。
