Shi Zi-Yi, Bao Zhong, Jiang Yong, Tu Peng-Fei
School of Pharmaceutical Sciences, Modern Research Center for Traditional Chinese Medicine, Peking University Health Science Center, Beijing 100083, China.
Zhongguo Zhong Yao Za Zhi. 2007 May;32(9):779-83.
To establish the quantitative methods for calycosin glycoside and formononetin in Radix Astragali, and the samples from different sources were analyzed, in order to supply the basis for the quality control of Radix Astragali.
The content of calycosin glycoside and formononetin in 59 samples of Radix Astragali from eight with different provinces was analyzed by HPLC-DAD.
The contents of calycosin glycoside and formononetin in Radix Astragali from different sources, with different cultivating method or in different ages differed markedly, and the results showed that the quality of samples from Shannxi, Innermongolia and Shanxi were better than other sources, and the semi-wild samples were better than other cultiving samples, moreover the shorter age, the better quality.
This simple, accurate and reproducible method could use to determine the contents of isoflavanoids in Radix Astragali.
建立黄芪中毛蕊异黄酮葡萄糖苷和芒柄花素的定量分析方法，并对不同来源的黄芪样品进行分析，为黄芪的质量控制提供依据。
采用高效液相色谱-二极管阵列检测器（HPLC-DAD）法测定来自八个不同省份的59份黄芪样品中毛蕊异黄酮葡萄糖苷和芒柄花素的含量。
不同来源、不同栽培方式及不同年份的黄芪中毛蕊异黄酮葡萄糖苷和芒柄花素的含量差异显著，结果表明陕西、内蒙古和山西的样品质量优于其他来源，半野生样品优于其他栽培样品，且年份越短，质量越好。
该方法简便、准确、重现性好，可用于测定黄芪中异黄酮类成分的含量。
