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短链脂肪酸对绵羊瘤胃壁氨吸收的影响。

Influence of short-chain fatty acids on ammonia absorption across the rumen wall in sheep.

作者信息

Bödeker D, Shen Y, Kemkowski J, Höller H

机构信息

Department of Physiology, School of Veterinary Medicine, Hannover, Germany.

出版信息

Exp Physiol. 1992 Mar;77(2):369-76. doi: 10.1113/expphysiol.1992.sp003597.

DOI:10.1113/expphysiol.1992.sp003597
PMID:1581065
Abstract

The effects of short-chain fatty acids (SCFA) on ammonia net absorption from the sheep rumen in vivo and on ammonia transport across rumen wall mucosa in vitro were studied. Ammonia net absorption was directly, though in a non-linear manner, correlated with the SCFA concentration in the artificial rumen fluid. Almost 70% of total ammonia absorption was dependent upon the presence of SCFA when 12 mmol l-1 ammonia and 67.5 mmol l-1 SCFA were present. Lactic acid was ineffective. Incubation experiments showed that mucosal disappearance and serosal appearance of ammonia were reduced by 38% and 32%, respectively, when SCFA (63 mmol l-1) were replaced by lactic acid. The SCFA effect was independent of the type of SCFA used. In part of the experiments up to 54% of the ammonia taken up by the tissue was not recovered in the serosal incubation solution and must have been metabolized in the mucosa.

摘要

研究了短链脂肪酸(SCFA)对绵羊瘤胃体内氨净吸收以及体外氨跨瘤胃壁黏膜转运的影响。氨净吸收与人工瘤胃液中SCFA浓度呈直接但非线性的相关关系。当存在12 mmol l-1氨和67.5 mmol l-1 SCFA时,总氨吸收的近70%依赖于SCFA的存在。乳酸无效。孵育实验表明,当用乳酸替代SCFA(63 mmol l-1)时,氨的黏膜消失和浆膜出现分别减少了38%和32%。SCFA的作用与所用SCFA的类型无关。在部分实验中,组织摄取的氨中高达54%未在浆膜孵育溶液中回收,必定已在黏膜中代谢。

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