大鼠骨髓基质细胞中的胶质细胞源性神经营养因子

Glial cell line-derived neurotrophic factor in bone marrow stromal cells of rat.

作者信息

Ye Min, Chen Shengdi, Wang Xijin, Qi Chen, Lu Guoqiang, Liang Liang, Xu Jieyi

机构信息

Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai, Second Medical University, Shanghai 200025, China.

出版信息

Neuroreport. 2005 Apr 25;16(6):581-4. doi: 10.1097/00001756-200504250-00013.

DOI:10.1097/00001756-200504250-00013

PMID:15812312

Abstract

To investigate the capability of Sprague-Dawley rat bone marrow stromal cells to secrete glial cell line-derived neurotrophic factors (GDNF), we detected expression of GDNF messenger RNA and protein in bone marrow stromal cells of Sprague-Dawley rats by reverse-transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA), respectively. The GDNF messenger RNA and protein were detected in culture medium and total cell protein when bone marrow stromal cells were cultured for 3 days. The levels of GDNF in culture medium and total cell protein increased gradually after 3, 7 and 10 days of culture. Rat bone marrow stromal cells have the potential to secrete GDNF. Furthermore, the ability of secretion is determined by the surrounding microenvironment and self-growth status.

摘要

为研究Sprague-Dawley大鼠骨髓基质细胞分泌胶质细胞源性神经营养因子（GDNF）的能力，我们分别通过逆转录聚合酶链反应和酶联免疫吸附测定（ELISA）检测了Sprague-Dawley大鼠骨髓基质细胞中GDNF信使核糖核酸（mRNA）和蛋白的表达。当骨髓基质细胞培养3天时，在培养基和总细胞蛋白中检测到了GDNF信使核糖核酸和蛋白。培养3、7和10天后，培养基和总细胞蛋白中GDNF的水平逐渐升高。大鼠骨髓基质细胞具有分泌GDNF的潜力。此外，分泌能力由周围微环境和自身生长状态决定。