Manteuffel-Cymborowska M, Chmurzynska W, Grzelakowska-Sztabert B
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
Biochim Biophys Acta. 1992 Apr 22;1116(2):166-72. doi: 10.1016/0304-4165(92)90113-9.
Exogenous administration of testosterone produced several metabolic tissue-specific changes in female mouse kidneys, but not in the liver. The hormone induced ornithine decarboxylase (ODC) activity, and also profoundly influenced metabolism of S-adenosylmethionine (AdoMet). Therefore, the activity of the AdoMet-synthesizing enzyme (AdoMet synthetase) and of cystathionine synthase, which commits homocysteine irreversibly to the transsulfuration pathway, were significantly increased. In contrast to the level of AdoMet in the liver the renal level of this metabolite was augmented, whereas the level of S-adenosylhomocysteine (AdoHcy) did not change. This resulted in an increase of the AdoMet/AdoHcy ratio. In testosterone-treated mice, pulse-labelled with [methyl-14C]methionine, the radioactivity recovered in the kidneys doubled, but in the liver remained the same. The rise in radioactivity recovered occurred mainly in TCA-soluble compounds and lipids, and to a smaller extent, in proteins and nucleic acids.
外源性给予睾酮会在雌性小鼠肾脏中产生多种代谢组织特异性变化，但在肝脏中则不会。该激素诱导鸟氨酸脱羧酶（ODC）活性，并且还深刻影响S-腺苷甲硫氨酸（AdoMet）的代谢。因此，将同型半胱氨酸不可逆地导向转硫途径的AdoMet合成酶和胱硫醚合酶的活性显著增加。与肝脏中AdoMet的水平相反，该代谢物在肾脏中的水平升高，而S-腺苷同型半胱氨酸（AdoHcy）的水平没有变化。这导致AdoMet/AdoHcy比值增加。在用[甲基-14C]甲硫氨酸脉冲标记的睾酮处理的小鼠中，肾脏中回收的放射性增加了一倍，但肝脏中的放射性保持不变。回收的放射性增加主要发生在三羧酸循环（TCA）可溶性化合物和脂质中，在较小程度上发生在蛋白质和核酸中。
