RNA寡核苷酸中核糖无碱基位点的光化学生成。

Photochemical generation of ribose abasic sites in RNA oligonucleotides.

作者信息

Trzupek John D, Sheppard Terry L

机构信息

Department of Chemistry and The Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.

出版信息

Org Lett. 2005 Apr 14;7(8):1493-6. doi: 10.1021/ol050120h.

DOI:10.1021/ol050120h

PMID:15816735

Abstract

[reaction: see text] A general method for the photochemical generation of ribose abasic sites within RNA oligonucleotides is reported. Photochemically caged nucleoside phosphoramidite analogues were prepared and incorporated into RNA oligonucleotides by automated RNA synthesis. Irradiation of the modified RNA at 350 nm efficiently produced ribose abasic sites at specific sites within RNA sequences. The current approach offers a chemical route to RNA abasic lesions for RNA biochemical studies.

摘要

[反应：见正文] 报道了一种在RNA寡核苷酸内光化学生成核糖无碱基位点的通用方法。制备了光化学笼蔽的核苷亚磷酰胺类似物，并通过自动RNA合成将其掺入RNA寡核苷酸中。在350 nm处照射修饰后的RNA，能在RNA序列内的特定位点高效产生核糖无碱基位点。目前的方法为RNA生化研究中的RNA无碱基损伤提供了一条化学途径。