Kuo H-T, Kuo M-C, Chiu Y-W, Chang J-M, Guh J-Y, Chen H-C
Kaohsiung Medical University, Kaohsiung, Taiwan.
Eur J Clin Invest. 2005 Apr;35(4):245-50. doi: 10.1111/j.1365-2362.2005.01488.x.
Evidence suggests an increase in oxidative stress in patients with chronic kidney disease, as glomerulosclerosis is the prerequisite for chronic kidney disease; whether the oxidative stress already exists early on is not known.
In this study we measured the plasma and urinary levels of malondialdehyde (MDA), the end product of lipid peroxidation, and assessed the immunoreactivity of MDA and superoxide dismutase (SOD) in glomeruli of patients and rats with primary focal segmental glomerulosclerosis (FSGS), and compared our findings with those of minimal change disease (MCD) and normal controls (NC).
Our results showed that plasma MDA level was significantly increased in patients with FSGS compared with both patients with MCD and normal controls. The urinary MDA level was also significantly increased and was significantly correlated with plasma MDA level in patients with FSGS. The immunostaining for glomerular MDA and SOD was significantly higher in the patients with FSGS than in either the patients with MCD or NC, and was also significantly higher in rats with puromycin aminonucleoside (PAN)-induced FSGS than in rats with MCD. Glomerular MDA level was significantly correlated with the degree of glomerulosclerosis in the patients with FSGS.
Our data suggest that oxidative stress occurs early on before the onset of renal failure, and may play an important role in the pathogenesis of glomerulosclerosis.
有证据表明，慢性肾病患者的氧化应激增加，因为肾小球硬化是慢性肾病的先决条件；氧化应激是否在早期就已存在尚不清楚。
在本研究中，我们测量了脂质过氧化终产物丙二醛（MDA）的血浆和尿液水平，并评估了原发性局灶节段性肾小球硬化（FSGS）患者及大鼠肾小球中MDA和超氧化物歧化酶（SOD）的免疫反应性，并将我们的研究结果与微小病变肾病（MCD）患者及正常对照（NC）进行比较。
我们的结果显示，与MCD患者和正常对照相比，FSGS患者的血浆MDA水平显著升高。FSGS患者的尿MDA水平也显著升高，且与血浆MDA水平显著相关。FSGS患者肾小球MDA和SOD的免疫染色显著高于MCD患者或NC，嘌呤霉素氨基核苷（PAN）诱导的FSGS大鼠肾小球MDA和SOD的免疫染色也显著高于MCD大鼠。FSGS患者肾小球MDA水平与肾小球硬化程度显著相关。
我们的数据表明，氧化应激在肾衰竭发生之前就已早期出现，并可能在肾小球硬化的发病机制中起重要作用。
