Pitsikas Nikolaos, Tsitsirigou Stavroula, Zisopoulou Styliani, Sakellaridis Nikolaos
Department of Pharmacology, School of Medicine, University of Thessaly, 22 Papakiriazi Str., 412-22 Larissa, Greece.
Behav Brain Res. 2005 Apr 30;159(2):287-93. doi: 10.1016/j.bbr.2004.11.007. Epub 2004 Dec 13.
Functional activation of the 5-HT1A receptor inhibits cognition, although discrepant findings have also been reported. The present study was designed to investigate the role of the 5-HT1A receptor on recognition memory in the rat. For this purpose, the effects induced by the 5-HT1A agonist R-(+)-8-hydroxy-2-(di-n-propylamino)tetralin hydrobromide (8-OH-DPAT) and the 5-HT1A antagonist WAY 100635 on memory were evaluated by using the object recognition task. In addition, the possible involvement of the nitrergic system on 5-HT1A receptor's effects was also assessed by using the same behavioral procedure. In the first dose-response study, post-training administration of 8-OH-DPAT (0.1 and 0.3 mg/kg, subcutaneously (s.c.)) dose-dependently impaired animals' performance in this test. WAY 100635 (0.3 and 1 mg/kg, intraperitoneally (i.p.)) successfully antagonized these 8-OH-DPAT-induced performance deficits. The NO donor molsidomine (2 and 4 mg/kg, i.p.) counteracted cognition deficits produced by the highest dose of 8-OH-DPAT (0.3 mg/kg). Our findings indicate (a) that the 5-HT1A receptor is involved in recognition memory, and (b) that a NO component modulates the effects of the 5-HT1A receptor on learning and memory.
5-HT1A 受体的功能激活会抑制认知，尽管也有不一致的研究结果报道。本研究旨在探讨 5-HT1A 受体在大鼠识别记忆中的作用。为此，通过物体识别任务评估了 5-HT1A 激动剂 R-(+)-8-羟基-2-(二正丙基氨基)四氢萘溴化物（8-OH-DPAT）和 5-HT1A 拮抗剂 WAY 100635 对记忆的影响。此外，还通过相同的行为学程序评估了硝化系统对 5-HT1A 受体作用的可能参与情况。在第一项剂量反应研究中，训练后皮下注射 8-OH-DPAT（0.1 和 0.3 mg/kg）剂量依赖性地损害了动物在该测试中的表现。腹腔注射 WAY 100635（0.3 和 1 mg/kg）成功拮抗了这些由 8-OH-DPAT 诱导的表现缺陷。NO 供体吗多明（2 和 4 mg/kg，腹腔注射）抵消了最高剂量 8-OH-DPAT（0.3 mg/kg）产生的认知缺陷。我们的研究结果表明：（a）5-HT1A 受体参与识别记忆；（b）NO 成分调节 5-HT1A 受体对学习和记忆的作用。
