Law E, Gilvarry U, Lynch V, Gregory B, Grant G, Clynes M
National Cell and Tissue Culture Centre/Bioresearch Ireland, Dublin.
Cancer Genet Cytogenet. 1992 Apr;59(2):111-8. doi: 10.1016/0165-4608(92)90204-l.
This paper presents a cytogenetic analysis of two established but early-passage (passages 5 and 18) cell lines derived from histologically similar, poorly differentiated lymph node metastases of squamous cell carcinoma of the lung. The cell lines showed 3 shared marker chromosomes, del(1)(q11), del(2)(p11.1) and del(2)(q11.1). One of the lines (DLKP) had 8 additional markers including structural rearrangements such as translocations and isochromosomes. Five additional markers (including two deletions of chromosome 3) were found in DLRP. A notable feature of DLRP was the high incidence of telomeric association evident in the majority of metaphase plates. Over-representation of chromosome 7 was a characteristic feature of metaphases derived from DLKP, and identification of i(21q) in this cell line was an unusual finding. The results indicate significant cytogenetic heterogeneity between these early-passage cell lines derived from two apparently histologically similar tumors.
本文呈现了对两个已建立但传代较早（第5代和第18代）的细胞系的细胞遗传学分析，这两个细胞系源自组织学相似的低分化肺鳞状细胞癌淋巴结转移灶。这些细胞系显示出3条共同的标记染色体，即del(1)(q11)、del(2)(p11.1)和del(2)(q11.1)。其中一个细胞系（DLKP）还有8条额外的标记染色体，包括易位和等染色体等结构重排。在DLRP中发现了另外5条标记染色体（包括两条3号染色体缺失）。DLRP的一个显著特征是在大多数中期板中明显可见端粒关联的高发生率。7号染色体的过度代表是源自DLKP的中期相的一个特征，并且在该细胞系中鉴定出i(21q)是一个不寻常的发现。结果表明，源自两个明显组织学相似肿瘤的这些早期传代细胞系之间存在显著的细胞遗传学异质性。
