Petersen I, Bujard M, Petersen S, Wolf G, Goeze A, Schwendel A, Langreck H, Gellert K, Reichel M, Just K, du Manoir S, Cremer T, Dietel M, Ried T
Institute of Pathology, University Hospital Charité, Berlin, Germany.
Cancer Res. 1997 Jun 15;57(12):2331-5.
Comparative genomic hybridization was used to screen 25 adenocarcinomas and 25 squamous cell carcinomas of the lung for chromosomal imbalances. DNA copy number decreases common to both entities were observed on chromosomes 1p, 3p, 4q, 5q, 6q, 8p, 9p, 13q, 18q, and 21q. Similarly, DNA gains were observed for chromosomes 5p, 8q, 11q13, 16p, 17q, and 19q. Adenocarcinomas showed more frequently DNA overrepresentations of chromosome 1q and DNA losses on chromosomes 3q, 9q, 10p, and 19, whereas squamous cell carcinomas were characterized by increased overrepresentations of chromosome 3q and 12p as well as deletions of 2q. For the first time, we used a histogram representation and statistical analysis to evaluate the differences between both tumor groups. In particular, the overrepresentation of the chromosomal band 1q23 and the deletion at 9q22 were significantly associated with adenoid differentiation, whereas the DNA loss of chromosomal band 2q36-37 and the overrepresentations at 3q21-22 and 3q24-qter were statistically significant markers for the squamous cell type. The study strengthens the notion that different tumor subgroups of the respiratory tract are characterized by distinct patterns of chromosomal alterations.
采用比较基因组杂交技术筛查25例肺腺癌和25例肺鳞状细胞癌的染色体失衡情况。在1号染色体短臂、3号染色体短臂、4号染色体长臂、5号染色体长臂、6号染色体长臂、8号染色体短臂、9号染色体短臂、13号染色体长臂、18号染色体长臂和21号染色体长臂上观察到两种肿瘤共有的DNA拷贝数减少。同样，在5号染色体短臂、8号染色体长臂、11号染色体长臂13区、16号染色体短臂、17号染色体长臂和19号染色体长臂上观察到DNA增加。腺癌更常见的是1号染色体长臂DNA过度代表以及3号染色体长臂、9号染色体长臂、10号染色体短臂和19号染色体上的DNA缺失，而鳞状细胞癌的特征是3号染色体长臂和12号染色体短臂过度代表增加以及2号染色体缺失。我们首次使用直方图表示法和统计分析来评估两个肿瘤组之间的差异。特别是，染色体带1q23的过度代表和9q22处的缺失与腺样分化显著相关，而染色体带2q36 - 37的DNA缺失以及3q21 - 22和3q24 - qter处的过度代表是鳞状细胞类型的统计学显著标志物。该研究强化了这样一种观念，即呼吸道不同肿瘤亚组具有不同的染色体改变模式。
