Manjanaik B, Umesha K R, Karunasagar Indrani, Karunasagar Iddya
Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India.
Dis Aquat Organ. 2005 Feb 28;63(2-3):255-9. doi: 10.3354/dao063255.
The prevalence of hepatopancreatic parvovirus (HPV) in wild penaeid shrimp samples from India was studied by nested polymerase chain reaction (PCR) using primers designed in our laboratory. The virus could be detected in 9 out of 119 samples by non-nested PCR. However, by nested PCR 69 out of 119 samples were positive. The PCR results were confirmed by hybridization with digoxigenin-labelled DNA probe. Shrimp species positive by non-nested PCR included Penaeus monodon, Penaeus indicus and Penaeus semisulcatus and by nested PCR Parapenaeopsis stylifera, Penaeus japonicus, Metapenaeus monoceros, M. affinis, M. elegans, M. dobsoni, M. ensis and Solenocera choprai. This is the first report on the prevalence of HPV in captured wild shrimp from India.
使用我们实验室设计的引物，通过巢式聚合酶链反应（PCR）研究了印度野生对虾样本中肝胰腺细小病毒（HPV）的流行情况。通过非巢式PCR，在119个样本中的9个样本中检测到了该病毒。然而，通过巢式PCR，119个样本中有69个呈阳性。PCR结果通过与地高辛标记的DNA探针杂交得到证实。非巢式PCR呈阳性的虾种包括斑节对虾、印度对虾和近缘新对虾，巢式PCR呈阳性的有刀额新对虾、日本对虾、独角新对虾、亲缘新对虾、秀丽新对虾、多氏新对虾、殷氏新对虾和乔氏赤虾。这是关于印度捕获的野生虾中HPV流行情况的首次报告。
