Rønneseth Anita, Pettersen Eirin Fausa, Wergeland Heidrun I
Department of Biology, University of Bergen, Bergen High-Technology Center, Norway.
Fish Shellfish Immunol. 2005 Sep;19(3):229-39. doi: 10.1016/j.fsi.2004.12.005.
Using flow cytometry and leucocyte specific monoclonal antibodies, neutrophils and B-cells were studied in blood and head kidney from wild strains of Atlantic salmon. The strains were Vosso and Blege, being an anadromous and landlocked strain, respectively. Smoltification was induced using a simulated natural photoperiod and sampling was performed monthly for 6 months and ended for the Blege strain at the time of seawater transfer while samples were collected from the Vosso strain after 4 weeks in seawater. Throughout the observation period, the mean proportions of neutrophils in both head kidney leucocytes (HKL) and peripheral blood leucocytes (PBL), were highest for the Vosso strain. The opposite was observed for B-cells where the Blege strain had higher or similar mean proportions compared to the Vosso strain. There were some differences between HKL and PBL. The mean proportion of neutrophils was always higher in HKL than in PBL and the mean proportion of B-cells was higher in PBL than in HKL. The fluctuations during the observation period, in the proportions of B-cells and neutrophils of the analysed cell population, showed mainly the same pattern in both strains. Differences between the strains were observed at various times in the mean of total number of leucocytes per gram head kidney and per millilitre of blood. The fluctuations throughout the experimental period in total numbers of leucocytes in head kidney and blood followed mainly the same pattern in both strains. The results of the leucocyte analyses suggest that there are differences between the anadromous and landlocked strains with respect to what cell type is present in highest proportion in the leucocyte samples from head kidney and blood. The striking similarity between the strains is the profiles of proportions of B-cells and neutrophils in HKL and PBL during the smoltification period.
利用流式细胞术和白细胞特异性单克隆抗体，对大西洋鲑野生菌株血液和头肾中的中性粒细胞和B细胞进行了研究。这些菌株分别是Vosso和Blege，前者为溯河洄游型菌株，后者为陆封型菌株。采用模拟自然光周期诱导鲑鱼发生银化，每月采样一次，持续6个月，Blege菌株在海水转移时结束采样，而Vosso菌株在海水养殖4周后采集样本。在整个观察期内，Vosso菌株的头肾白细胞（HKL）和外周血白细胞（PBL）中中性粒细胞的平均比例最高。B细胞的情况则相反，Blege菌株的平均比例高于或与Vosso菌株相似。HKL和PBL之间存在一些差异。HKL中中性粒细胞的平均比例始终高于PBL，而PBL中B细胞的平均比例高于HKL。在观察期内，分析细胞群体中B细胞和中性粒细胞比例的波动在两种菌株中主要呈现相同的模式。在每克头肾和每毫升血液中白细胞总数的平均值方面，不同时间观察到了菌株之间的差异。在整个实验期内，头肾和血液中白细胞总数的波动在两种菌株中主要遵循相同的模式。白细胞分析结果表明，在头肾和血液的白细胞样本中，溯河洄游型和陆封型菌株在比例最高的细胞类型方面存在差异。两种菌株之间显著的相似之处在于银化期HKL和PBL中B细胞和中性粒细胞的比例分布情况。
