Pettersen Eirin F, Bjørløw Inge, Hagland Trude J, Wergeland Heidrun I
Department of Biology, University of Bergen, P.O. Box 7800, N-5020 Bergen, Norway.
Vet Immunol Immunopathol. 2005 Jun 15;106(1-2):65-76. doi: 10.1016/j.vetimm.2005.01.001.
Using monoclonal antibodies (mAb) and flow cytometry, the distribution of immunoglobulin positive (Ig+) cells and neutrophils in isolated peripheral blood leucocytes (PBL) and head kidney leucocytes (HKL) from Atlantic salmon under-yearling out-of-season smolts was studied in the post-smolt period from 0 to 14 weeks after seawater transfer. A temperature acclimation was performed in freshwater, resulting in four groups maintained at a water temperature of 18, 14, 10 and 6 degrees C, respectively. The temperature for each group was kept constant for the remaining period and all groups were reared under a simulated natural photoperiod regime (60 degrees 25'N). Sampling of eight fish from each temperature group was performed at regular intervals from 0 to 14 weeks after seawater transfer, starting the day after transfer (week 0). The seawater temperature influenced the distribution of the leucocyte populations, and the effect was most prominent in PBL. The lower rearing temperature (6 degrees C) resulted in higher percentages of Ig+ cells in PBL compared to fish reared at the other temperatures. The high temperature (18 degrees C) resulted in higher proportions of neutrophils and lower proportions of Ig+ cells in PBL compared to fish from the other temperature groups. The observed differences were consistent throughout the 14-week experimental period. While the present study indicate that rearing water temperature influence the distribution of leucocytes in blood of Atlantic salmon post-smolts, the proportions of HKL populations do not seem to be dependent on temperature to the same extent. Comparing the temperature groups, no clear differences in the percentages of Ig+ cells and neutrophils in HKL were observed. Likewise, no evident time-related changes in the leucocyte profiles within each temperature group could be observed during the studied post-smolt period. Significantly, the results could indicate that the post-smolts reared at a temperature of 18 degrees C experienced thermal stress or a non-optimal environment.
利用单克隆抗体（mAb）和流式细胞术，研究了在海水转移后0至14周的后洄游期，从大西洋鲑未成熟的反季节洄游幼鱼中分离出的外周血白细胞（PBL）和头肾白细胞（HKL）中免疫球蛋白阳性（Ig+）细胞和中性粒细胞的分布情况。在淡水中进行温度驯化，形成四组，分别维持在18、14、10和6摄氏度的水温下。每组的水温在剩余时间内保持恒定，所有组均在模拟自然光照周期（北纬60度25分）下饲养。从每个温度组中选取8条鱼，在海水转移后0至14周定期取样，从转移后的第二天（第0周）开始。海水温度影响白细胞群体的分布，且这种影响在PBL中最为显著。与在其他温度下饲养的鱼相比，较低的饲养温度（6摄氏度）导致PBL中Ig+细胞的百分比更高。与其他温度组的鱼相比，高温（18摄氏度）导致PBL中中性粒细胞比例更高，Ig+细胞比例更低。在整个14周的实验期内，观察到的差异是一致的。虽然本研究表明饲养水温会影响大西洋鲑后洄游幼鱼血液中白细胞的分布，但HKL群体的比例似乎在相同程度上并不依赖于温度。比较各温度组，未观察到HKL中Ig+细胞和中性粒细胞百分比的明显差异。同样，在研究的后洄游期内，每个温度组的白细胞谱中也未观察到明显的时间相关变化。值得注意的是，结果可能表明在18摄氏度温度下饲养的后洄游幼鱼经历了热应激或非最佳环境。
