Ueberrueck Torsten, Tautenhahn Joerg, Meyer Lutz, Kaufmann Olaf, Lippert Hans, Gastinger Ingo, Wahlers Thorsten
Freidrich-Schiller-University, Department of Cardiothoracic and Vascular Surgery, Erlanger Allee 101, 07747 Jena, Germany.
J Surg Res. 2005 Apr;124(2):305-11. doi: 10.1016/j.jss.2004.10.021.
Evaluation of the pig and sheep models for biocompatibility investigations of vascular prostheses (VP).
Comparative analysis of animal experimental investigations involving two different animal models.
Commercially available polyester vascular prostheses (PET-VP) were implanted into two different animal models (infrarenal porcine aorta and ovine carotid artery). The costs, surgical handling, patency rate, and healing on the basis of macroscopic, microscopic, and immunohistochemical criteria were analyzed over a period of 3 months.
Handling and operating times (63 +/- 10 versus 76 +/- 16 min; P = 0.125) did not differ significantly. The cost of the two animal models was comparable. Integration of the VP was complete in the sheep model, but varied in the pig model (two complete, four incomplete). Complete endothelialization of all VPs was observed in the pig, which contrasted with the sheep with complete (circular) endothelialization only in the region of the anastomosis. The thickness of neointima in the region of the anastomosis differed insignificantly; immunohistochemically, only periprosthetic Ki67 was significantly reduced (28.7 +/- 9.9 versus 6 +/- 0.9%; P = 0.002) in the sheep.
In the porcine model, extremely good endothelialization of the VP was observed, with formation of a rapid neointimal hyperplasia. The ovine model was characterized by the fact that postoperative follow-up investigations were easy to perform. Complete endothelialization was not observed.
评估猪和羊模型在血管假体（VP）生物相容性研究中的应用。
对涉及两种不同动物模型的动物实验研究进行比较分析。
将市售聚酯血管假体（PET-VP）植入两种不同动物模型（肾下腹主动脉猪模型和颈动脉羊模型）。在3个月的时间内，基于宏观、微观和免疫组织化学标准，分析成本、手术操作、通畅率和愈合情况。
操作和手术时间（63±10分钟对76±16分钟；P = 0.125）无显著差异。两种动物模型的成本相当。VP在羊模型中完全整合，但在猪模型中有所不同（两个完全整合，四个不完全整合）。在猪模型中观察到所有VP均完全内皮化，而在羊模型中仅在吻合区域观察到完全（环状）内皮化。吻合区域新生内膜厚度差异不显著；免疫组织化学分析显示，仅羊模型中假体周围的Ki67显著降低（28.7±9.9%对6±0.9%；P = 0.002）。
在猪模型中，观察到VP具有极好的内皮化，伴有快速的新生内膜增生。羊模型的特点是术后随访调查易于进行，但未观察到完全内皮化。
