Lozano Francisco S, Rodríguez José M, García-Criado Francisco J, Gonzalez-Porras Jose R, Sanchez-Guijo Fermin M, Sanchez-Conde Pilar, García-Sanchez Jose E
Department of Surgery, Faculty of Medicine, Universidad de Salamanca, University Hospital of Salamanca, Avda. Alfonso X El Sabio s/n, 37007 Salamanca, Spain.
World J Surg. 2008 Apr;32(4):642-7. doi: 10.1007/s00268-007-9346-2.
The aim of this study was to design an aggressive nonlethal animal model that would simulate surgical treatment of the abdominal aorta with a view to studying the systemic inflammatory response. Fourteen pigs were subjected to two sequential experiments. Experiment A was performed to determine the response to two degrees of hemorrhage: (A1) 40% bleeding; and (A2) 60% bleeding over 15 minutes followed by midline laparotomy and aortic dissection. Experiment B included two methods of aortic repair: (B1) aortic resection and replacement with a prosthesis; and (B2) aortic bypass without aortic resection. In the latter two groups, suprarenal aortic cross-clamping was placed for 30 minutes after a 40% hemorrhage. We analyzed various inflammatory markers and mortality. The 40% bleeding (vs. 60%) elicited a smaller decrease in mean arterial pressure (110 +/- 6 vs. 89 +/- 9 mmHg) but did not cause irreversible shock or mortality. After the 40% hemorrhage, the B1 aortic repair caused two cases of paraplegia. We have developed a model to study the combined effect of bleeding and aortic cross-clamping.
本研究的目的是设计一种侵袭性非致死性动物模型，该模型可模拟腹主动脉的外科治疗，以便研究全身炎症反应。14头猪接受了两个连续的实验。实验A旨在确定对两种程度出血的反应：（A1）40%失血；以及（A2）15分钟内60%失血，随后进行中线剖腹术和主动脉剥离。实验B包括两种主动脉修复方法：（B1）主动脉切除并用假体置换；以及（B2）不进行主动脉切除的主动脉旁路移植术。在后两组中，40%失血后进行30分钟的肾上主动脉交叉钳夹。我们分析了各种炎症标志物和死亡率。40%失血（与60%相比）引起的平均动脉压下降较小（110±6与89±9 mmHg），但未导致不可逆休克或死亡。40%失血后，B1主动脉修复导致两例截瘫。我们已经开发出一种模型来研究出血和主动脉交叉钳夹的联合效应。
