Domenico P, Salo R J, Straus D C, Hutson J C, Cunha B A
Infectious Disease Division, Winthrop-University Hospital, Mineola, New York 11501.
Infection. 1992 Mar-Apr;20(2):66-72. doi: 10.1007/BF01711065.
After treatment of encapsulated Klebsiella pneumoniae with salicylate or bismuth compounds, phagocytic uptake by human peripheral white blood cells or rat alveolar macrophages was assessed. Without salicylate pretreatment of bacteria, a 30-60% net increase in viable bacteria resulted in phagocytic assays after a 1 hour incubation. With salicylate pretreatment, dose-related decreases in bacterial counts were seen, achieving a maximal reduction of 60% with 240 microM salicylate pretreatment. Bacterial variants producing less capsule were more serum sensitive and more readily phagocytosed. Micrographs of Giemsa-stained cells revealed phagocytic uptake of multiple bacteria after salicylate pretreatment, but virtually no uptake of untreated bacteria. Opsonization with polyclonal antiserum decreased bacterial cell counts by 20% without and by 90% with salicylate pretreatment of bacteria. Pretreatment of bacteria with bismuth salts also enhanced opsonophagocytosis of encapsulated bacteria. Thus, agents known to reduce capsule expression in K. pneumoniae also enhance phagocytic uptake of bacteria.
在用水杨酸盐或铋化合物处理包膜肺炎克雷伯菌后，评估了人外周血白细胞或大鼠肺泡巨噬细胞的吞噬摄取情况。在未对细菌进行水杨酸盐预处理的情况下，经过1小时孵育后，吞噬试验中活细菌净增加30%至60%。经过水杨酸盐预处理后，可见细菌计数呈剂量相关下降，水杨酸盐预处理浓度为240微摩尔时，细菌计数最大减少60%。产生较少荚膜的细菌变体对血清更敏感，更容易被吞噬。吉姆萨染色细胞的显微照片显示，经过水杨酸盐预处理后，细胞能吞噬多个细菌，但未处理的细菌几乎不被摄取。用多克隆抗血清进行调理，在未对细菌进行水杨酸盐预处理时，细菌细胞计数减少20%，而在对细菌进行水杨酸盐预处理时，细菌细胞计数减少90%。用铋盐预处理细菌也增强了包膜细菌的调理吞噬作用。因此，已知能降低肺炎克雷伯菌荚膜表达的药物也能增强细菌的吞噬摄取。
