Nilsson M, Hellström S, Hedlund U
Department of Anatomy, University of Umeå, Sweden.
Histochem J. 1992 Mar;24(3):166-9. doi: 10.1007/BF01047466.
To conduct SEM studies on epithelium containing mucus-producing cells it is essential to remove the mucus which normally obscures the epithelial surface. This study presents a method which effectively removes the covering layer of mucus in the rat middle ear. Healthy Sprague-Dawley rats were decapitated and the middle ears dissected free. Incubation and agitation of the middle ear specimens in hyaluronidase (50 IE ml-1) and/or glucosidase (8%) removed the mucus from the middle ear cavity without altering the surface structures. It was also revealed that substances such as polyvinyl-pyrrolidone (PVP) (used to increase the colloid osmotic pressure of, e.g., the fixative solution) must be omitted when preparing ciliated specimens for SEM.
要对含有黏液分泌细胞的上皮组织进行扫描电子显微镜（SEM）研究，去除通常会遮盖上皮表面的黏液至关重要。本研究提出了一种有效去除大鼠中耳黏液覆盖层的方法。将健康的斯普拉格-道利大鼠断头，分离出中耳。在透明质酸酶（50 IE/ml-1）和/或葡萄糖苷酶（8%）中孵育并搅动中耳标本，可去除中耳腔内的黏液，且不改变表面结构。研究还表明，在制备用于SEM的纤毛标本时，必须省略聚乙烯吡咯烷酮（PVP）等物质（用于增加例如固定液的胶体渗透压）。
