Hermansson A, Prellner K, Hellström S
Department of Oto-Rhino-Laryngology, University Hospital, Lund, Sweden.
Acta Otolaryngol. 1990 May-Jun;109(5-6):421-30. doi: 10.3109/00016489009125164.
To examine the effect of a transient episode of purulent AOM on the middle ear mucosa, an animal model was used. Histological examination was performed after inoculation of pneumococci into the rat middle ear. At 4, 8 and 12 days, and at 2 months after the inoculation, rats were sacrificed on each occasion and samples taken from specific areas of the middle ear mucosa. Structural alterations were noted at all mucosal sites. The normally flat epithelium had become more cuboidal or cylindrical, and numerous ciliated cells occurred in areas originally devoid of these cells. Epithelial cells penetrated the subepithelium forming gland-like structures. In the early phase, day 4 and day 8, inflammatory cells invaded the subepithelial layer except in the tympanic membrane. At 2 months, with the tympanic membrane otimicroscopically and histologically normalized, the structural changes of the mucosa still persisted indicating that a transient episode of pneumococcal otitis media can cause persisting mucosal changes.
为研究化脓性急性中耳炎短暂发作对中耳黏膜的影响，采用了动物模型。将肺炎球菌接种到大鼠中耳后进行组织学检查。在接种后的第4、8和12天以及2个月时，每次处死大鼠，并从中耳黏膜的特定区域取样。所有黏膜部位均观察到结构改变。正常扁平的上皮变得更加立方状或柱状，并且在原本没有这些细胞的区域出现了大量纤毛细胞。上皮细胞穿透上皮下层形成腺样结构。在早期，即第4天和第8天，除鼓膜外，炎性细胞侵入上皮下层。在2个月时，鼓膜在耳显微镜和组织学上恢复正常，但黏膜的结构变化仍然存在，这表明肺炎球菌性中耳炎的短暂发作可导致黏膜持续改变。
