Ji Yong Cheol, Kim Young Baeg, Park Seung Won, Hwang Sung Nam, Min Byung Kook, Hong Hyun Jong, Kwon Jeong Taik, Suk Jong Sik
Department of Neurosurgery, College of Medicine, Chung-Ang University, Seoul 156-755, Korea.
J Korean Med Sci. 2005 Apr;20(2):291-6. doi: 10.3346/jkms.2005.20.2.291.
In the present study, we investigated whether ginseng total saponins (GTSs) protect hippocampal neurons after experimental traumatic brain injury (TBI) in rats. A moderate-grade TBI was made with the aid of a controlled cortical impact (CCI) device set at a velocity of 3.0 m/sec, a deformation of 3.0 mm, and a compression time of 0.2 sec at the right parietal area for adult male Sprague-Dawley rats. Shamoperated rats that underwent craniectomy without impact served as controls. GTSs (100 and 200 mg/kg) or saline was injected intraperitoneally into the rats immediately post-injury. Twenty-four hours after the injury, the rats underwent neurological evaluation. Contusion volume and the number of hippocampal neurons were calculated with apoptosis evaluated by TUNEL staining. 24 hr post-injury, saline-injected rats showed a significant loss of neuronal cells in the CA2 region of the right hippocampus (53.4%, p<0.05) and CA3 (34.6%, p<0.05) compared with contralateral hippocampal region, a significant increase in contusion volume (34+/-8 microL), and significant increase in neurologic deficits compared with the GTSs groups. Treating rats with GTSs seemed to protect the CCI-induced neuronal loss in the hippocampus, decrease cortical contusion volume, and improve neurological deficits.
在本研究中，我们调查了人参总皂苷（GTSs）是否能在大鼠实验性创伤性脑损伤（TBI）后保护海马神经元。借助于可控皮质撞击（CCI）装置，对成年雄性Sprague-Dawley大鼠右侧顶叶区域造成中度TBI，撞击参数设置为速度3.0米/秒、变形3.0毫米、压缩时间0.2秒。接受开颅但未撞击的假手术大鼠作为对照。损伤后立即给大鼠腹腔注射GTSs（100和200毫克/千克）或生理盐水。损伤24小时后，对大鼠进行神经学评估。通过TUNEL染色评估凋亡情况，计算挫伤体积和海马神经元数量。损伤后24小时，与对侧海马区域相比，注射生理盐水的大鼠右侧海马CA2区（53.4%，p<0.05）和CA3区（34.6%，p<0.05）的神经元细胞显著丢失，挫伤体积显著增加（34±8微升），与GTSs组相比神经功能缺损显著增加。用GTSs治疗大鼠似乎能保护CCI诱导的海马神经元丢失，减少皮质挫伤体积，并改善神经功能缺损。
