Chen Xiao-Chun, Zhu Yuan-Gui, Zhu Li-An, Huang Chun, Chen Ying, Chen Li-Min, Fang Fang, Zhou Yi-Can, Zhao Chao-Hui
Fujian Institute of Geriatrics, the Affiliated Union Hospital of Fujian Medical University, No. 29, Xinquan Road, Fuzhou, PO 350001, China.
Eur J Pharmacol. 2003 Jul 18;473(1):1-7. doi: 10.1016/s0014-2999(03)01945-9.
In Parkinson's disease, neuroprotective therapy to rescue dopamine neurons has been proposed. Ginsenoside Rg1, one of the biologically active ingredients of ginseng, may be a candidate neuroprotective drug. In the present study, the mechanism underlying the neuroprotection provided by ginsenosde Rg1 was studied against apoptosis induced by exogenous dopamine in PC12 cells. Pretreatment with ginsenoside Rg1 markedly reduced the generation of dopamine-induced reactive oxygen species and the release of mitochondrial cytochrome c into the cytosol, and subsequently inhibited the activation of caspase-3. In addition, Rg1 pretreatment also reduced inducible nitric oxide (NO) synthase protein level and NO production. These results suggested that ginsenoside Rg1 may attenuate dopamine-induced apoptotic cell death through suppression of intracellular oxidative stress, and that it may rescue or protect dopamine neurons in Parkinson's disease.
在帕金森病中，已提出采用神经保护疗法来挽救多巴胺能神经元。人参皂苷Rg1是人参的生物活性成分之一，可能是一种潜在的神经保护药物。在本研究中，对人参皂苷Rg1提供神经保护作用以对抗PC12细胞中外源性多巴胺诱导的细胞凋亡的机制进行了研究。人参皂苷Rg1预处理显著减少了多巴胺诱导的活性氧生成以及线粒体细胞色素c释放到细胞质中，随后抑制了caspase-3的激活。此外，Rg1预处理还降低了诱导型一氧化氮合酶蛋白水平和一氧化氮生成。这些结果表明，人参皂苷Rg1可能通过抑制细胞内氧化应激来减轻多巴胺诱导的凋亡性细胞死亡，并且它可能挽救或保护帕金森病中的多巴胺能神经元。
