Ge Dongtao, Wu Dewang, Shi Wei, Ma Yuanyuan, Tian Xiangdong, Liang Pengfei, Zhang Qiqing
Biomedical Engineering Research Center, Medical College, Xiamen University, Xiamen 361005, People's Republic of China.
Biomed Mater. 2006 Sep;1(3):170-4. doi: 10.1088/1748-6041/1/3/012. Epub 2006 Aug 25.
Established methods for kidney dialysis do not work for liver failure because kidney dialysis removes only water-soluble toxins, while the liver normally removes albumin-bound toxins. In the present study, a polysulfone dialysis membrane with a -OH reactive group was prepared by hydrolyzing the chloromethylated polysulfone membrane, and the bovine serum albumin molecules were fixed into the membrane with 1,1'-carbonyldiimidazole activation. The content of albumin of the albumin-fixed membrane was 121.3 mg (g membrane)(-1). The albumin-fixed dialysis membranes were used to remove protein-bound toxins, bilirubin, from the bilirubin-albumin solution. The transfer rate of bilirubin of the albumin-fixed membrane was obviously higher compared to the normal dialysis membrane. The clearance of bilirubin with the albumin-fixed membrane was 49.8%. The albumin-fixed membrane can easily be regenerated by the bovine serum albumin and NaOH solution. Regeneration of the membrane suggested good mechanical and chemical stability, as well as good clearance of bilirubin. In addition, the effects of membrane thickness and bilirubin initial concentration on the removal of bilirubin were discussed.
已有的肾脏透析方法对肝衰竭不起作用，因为肾脏透析只能清除水溶性毒素，而肝脏通常能清除与白蛋白结合的毒素。在本研究中，通过水解氯甲基化聚砜膜制备了具有-OH反应基团的聚砜透析膜，并用1,1'-羰基二咪唑活化将牛血清白蛋白分子固定到膜中。固定白蛋白的膜中白蛋白含量为121.3 mg (g膜)(-1)。使用固定白蛋白的透析膜从胆红素-白蛋白溶液中去除与蛋白质结合的毒素胆红素。与普通透析膜相比，固定白蛋白的膜对胆红素的转运速率明显更高。固定白蛋白的膜对胆红素的清除率为49.8%。固定白蛋白的膜可以很容易地用牛血清白蛋白和氢氧化钠溶液再生。膜的再生表明其具有良好的机械和化学稳定性，以及良好的胆红素清除能力。此外，还讨论了膜厚度和胆红素初始浓度对胆红素去除的影响。
