Chen Tian-Xiang, Nie Hua-Li, Li Shu-Bai, Branford-White Christopher, Su Sai-Nan, Zhu Li-Min
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.
Colloids Surf B Biointerfaces. 2009 Aug 1;72(1):25-31. doi: 10.1016/j.colsurfb.2009.03.012. Epub 2009 Mar 27.
Novel membranes that involve the immobilization of Reactive Red 120 or Reactive Brown 10 as dye ligands were prepared. These were used in the purification of papain from papaya powder extracts. Papain adsorption capacities for the Red 120 and Brown 10 membranes were 143.6 mg/g and 107.3mg/g, respectively. The effectiveness of adsorption was demonstrated by Freundlich isotherm proficiency. The enzyme was eluted from the respective dye membranes using 1.0M NaCl at pH 6.0 and yields of over 80% were found for the Red 120-CS (chitosan)-nylon membrane whereas only a 50% recovery was possible using the Brown 10-CS-nylon membranes. It is concluded that Red 120-CS-nylon membranes could play an active role in the separation and purification of papain from crude extracts. This system has the potential to be developed for the commercial isolation of the protein.
制备了新型膜，其中固定了活性红120或活性棕10作为染料配体。这些膜用于从木瓜粉提取物中纯化木瓜蛋白酶。红色120膜和棕色10膜对木瓜蛋白酶的吸附容量分别为143.6 mg/g和107.3 mg/g。Freundlich等温线表明了吸附的有效性。在pH 6.0条件下，用1.0M NaCl从相应的染料膜上洗脱酶，红色120-壳聚糖-尼龙膜的产率超过80%，而棕色10-壳聚糖-尼龙膜的回收率仅为50%。结论是，红色120-壳聚糖-尼龙膜在从粗提取物中分离和纯化木瓜蛋白酶方面可以发挥积极作用。该系统有潜力用于蛋白质的商业分离。
