感染朊病毒疾病的动物和人类尿液中免疫球蛋白轻链的特征分析。

Characterization of light chain immunoglobulin in urine from animals and humans infected with prion diseases.

作者信息

Kariv-Inbal Zehavit, Halimi Michele, Dayan Yael, Engelstein Roni, Gabizon Ruth

机构信息

Department of Neurology, The Agnes Ginges Center for Human Neurogenetics, Hadassah University Hospital, Jerusalem 91120, Israel.

出版信息

J Neuroimmunol. 2005 May;162(1-2):12-8. doi: 10.1016/j.jneuroim.2004.12.013.

DOI:10.1016/j.jneuroim.2004.12.013

PMID:15833355

Abstract

The necessity of a non-invasive in-vivo test for prion diseases has become more apparent since the transmission of vCJD from the blood of a healthy individual incubating the disease. Here we show that prion urine comprises an array of protease resistant peptides, among them light chain immunoglobulin (LC). This was observed by sequencing gel bands comprising hamster urine samples, as well as by immunoblotting of similar samples with anti mouse IgG reagents for hamster samples, or with anti human IgG reagents for human samples. Our result suggests that urine samples from CJD patients can be identified by the presence of protease resistant proteins such as LC.

摘要

自从变异型克雅氏病（vCJD）从处于疾病潜伏期的健康个体血液中传播以来，对朊病毒疾病进行非侵入性体内检测的必要性变得更加明显。在此我们表明，朊病毒尿液包含一系列蛋白酶抗性肽，其中包括轻链免疫球蛋白（LC）。这是通过对包含仓鼠尿液样本的测序凝胶条带进行测序观察到的，也通过使用针对仓鼠样本的抗小鼠IgG试剂或针对人类样本的抗人IgG试剂对类似样本进行免疫印迹观察到的。我们的结果表明，克雅氏病（CJD）患者的尿液样本可通过存在诸如LC等蛋白酶抗性蛋白来识别。