利用聚合酶链反应检测炭疽芽孢杆菌的孢子
Detection of spores of Bacillus anthracis using the polymerase chain reaction.
作者信息
Carl M, Hawkins R, Coulson N, Lowe J, Robertson D L, Nelson W M, Titball R W, Woody J N
机构信息
Naval Medical Research Institute, Naval Medical Research and Development Command, Bethesda, Maryland.
出版信息
J Infect Dis. 1992 Jun;165(6):1145-8. doi: 10.1093/infdis/165.6.1145.
The polymerase chain reaction (PCR) was used to identify spores of Bacillus anthracis. By using an assay capable of amplifying a 1247-bp fragment from the gene that encodes the edema factor of B. anthracis, as few as 10(3) copies of a plasmid containing the edema factor gene and as few as 2 x 10(4) spores were detected. Subjecting the product of this PCR to a second PCR designed to amplify a 208-bp fragment nested within the 1247-bp product improved detection to a single plasmid copy per PCR and to two spores of B. anthracis per PCR.
聚合酶链反应（PCR）被用于鉴定炭疽芽孢杆菌的孢子。通过使用一种能够从编码炭疽芽孢杆菌水肿因子的基因中扩增出1247碱基对片段的检测方法，可检测到低至10³个含有水肿因子基因的质粒拷贝以及低至2×10⁴个孢子。将该PCR产物进行第二次PCR，该第二次PCR旨在扩增嵌套在1247碱基对产物内的208碱基对片段，从而将检测灵敏度提高到每个PCR检测单个质粒拷贝以及每个PCR检测两个炭疽芽孢杆菌孢子。
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