Lundgren B, Lundgren J D, Nielsen T, Mathiesen L, Nielsen J O, Kovacs J A
Department of Infectious Diseases, University of Copenhagen, Hvidovre Hospital, Denmark.
J Infect Dis. 1992 Jun;165(6):1151-5. doi: 10.1093/infdis/165.6.1151.
Antibody responses to a major purified human Pneumocystis carinii surface antigen (gp95) were determined by ELISA in human immunodeficiency virus (HIV)-infected patients. Serum IgG directed against gp95 was measured in 129 consecutive HIV-infected patients who underwent bronchoscopy for evaluation of pulmonary symptoms. Significantly more patients with P. carinii pneumonia (PCP) had detectable antibodies compared with HIV-infected patients without PCP and with HIV-negative controls (50 [66%] of 76 vs. 18 [34%] of 53 and 7 [35%] of 20, respectively; P less than .001), and the level of antibody response was higher (mean optical density ratio: 0.6 vs. 0.23 and 0.2, respectively; P less than .01). Changes in antibody response were investigated in 78 patients for whom serial serum samples taken around the time of bronchoscopy were available. Of the 47 patients with verified PCP, 20 (43%) mounted an antibody response, compared with only 1 (3%) of 31 patients without PCP (P less than .001). This patient had PCP on the basis of clinical criteria, including response to therapy. Thus, despite severe immunosuppression, a proportion of HIV-infected patients with PCP can mount a specific IgG-mediated antibody response to P. carinii.
通过酶联免疫吸附测定法（ELISA）检测了人类免疫缺陷病毒（HIV）感染患者对一种主要的纯化人卡氏肺孢子虫表面抗原（gp95）的抗体反应。在129例因肺部症状接受支气管镜检查的连续HIV感染患者中，检测了针对gp95的血清IgG。与未患卡氏肺孢子虫肺炎（PCP）的HIV感染患者及HIV阴性对照相比，患PCP的患者中可检测到抗体的比例显著更高（分别为76例中的50例[66%]、53例中的18例[34%]和20例中的7例[35%]；P<0.001），且抗体反应水平更高（平均光密度比值分别为0.6、0.23和0.2；P<0.01）。对78例在支气管镜检查前后采集了系列血清样本的患者的抗体反应变化进行了研究。在47例确诊为PCP的患者中，20例（43%）出现了抗体反应，而在31例未患PCP的患者中只有1例（3%）出现抗体反应（P<0.001）。该患者根据包括对治疗反应在内的临床标准被诊断为PCP。因此，尽管存在严重免疫抑制，但一部分患PCP的HIV感染患者能够对卡氏肺孢子虫产生特异性IgG介导的抗体反应。
