Peglow S L, Smulian A G, Linke M J, Pogue C L, Nurre S, Crisler J, Phair J, Gold J W, Armstrong D, Walzer P D
Department of Internal Medicine, University of Cincinnati College of Medicine, Ohio.
J Infect Dis. 1990 Feb;161(2):296-306. doi: 10.1093/infdis/161.2.296.
Serum antibodies to human Pneumocystis carinii antigens were measured in greater than 400 specimens from different population groups by the immunoblotting technique. Serologic responses varied during the first 2 years of life, but in children greater than or equal to 2 1/2 years and in adults antibodies to a 40-kDa band were found in greater than 85% of the specimens; antigens to bands of 66, 92, and 116 kDa were also detected frequently. The prevalence of serum antibodies in immunosuppressed patients varied at different institutions and was usually lower than that of healthy controls. Seven (41%) of 17 patients with single episodes of pneumocystosis and 13 (93%) of 14 patients with recurrent episodes followed sequentially developed active serum IgM and/or IgG antibody responses to the 40-kDa antigen. Serologic responses to P. carinii were also detected, though less frequently, by immunofluorescence. These data suggest that the 40-kDa antigen is a major marker of P. carinii infection and that immunoblotting is useful in measuring serum antibody responses to the organism in both normal and immunocompromised hosts.
采用免疫印迹技术检测了来自不同人群组的400多份标本中针对人卡氏肺孢子虫抗原的血清抗体。在生命的最初2年中血清学反应有所不同，但在2岁半及以上的儿童和成人中，超过85%的标本中发现了针对一条40 kDa条带的抗体；66 kDa、92 kDa和116 kDa条带的抗原也经常被检测到。免疫抑制患者血清抗体的流行率在不同机构有所不同，且通常低于健康对照。17名单发肺孢子虫病患者中有7名（41%），14名复发患者中有13名（93%）随后依次出现了针对40 kDa抗原的活性血清IgM和/或IgG抗体反应。通过免疫荧光也检测到了对卡氏肺孢子虫的血清学反应，不过频率较低。这些数据表明，40 kDa抗原是卡氏肺孢子虫感染的主要标志物，免疫印迹法在检测正常宿主和免疫受损宿主中针对该病原体的血清抗体反应方面很有用。
