Touboul David, Brunelle Alain, Halgand Frédéric, De La Porte Sabine, Laprévote Olivier
Laboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2301, F91198 Gif sur Yvette Cedex, France.
J Lipid Res. 2005 Jul;46(7):1388-95. doi: 10.1194/jlr.M500058-JLR200. Epub 2005 Apr 16.
Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has expanded very rapidly with the development of gold cluster ion sources (Au(3+)). It is now possible to acquire ion density maps (ion images) on a tissue section without any treatment and with a lateral resolution of few micrometers. In this article, we have taken advantage of this technique to study the degeneration/regeneration process in muscles of a Duchenne muscular dystrophy model mouse. Specific distribution of different lipid classes (fatty acids, triglycerides, phospholipids, tocopherol, coenzyme Q9, and cholesterol) allows us to distinguish three different regions on a mouse leg section: one is destroyed, another is degenerating (oxidative stress and deregulation of the phosphoinositol cycle), and the last one is stable. TOF-SIMS imaging shows the ability to localize directly on a tissue section a great number of lipid compounds that reflect the state of the cellular metabolism.
随着金团簇离子源（Au(3+)）的发展，飞行时间二次离子质谱（TOF-SIMS）成像技术得到了迅速发展。现在可以在未经任何处理的组织切片上获取离子密度图（离子图像），横向分辨率可达几微米。在本文中，我们利用这项技术研究了杜氏肌营养不良模型小鼠肌肉中的退化/再生过程。不同脂质类别（脂肪酸、甘油三酯、磷脂、生育酚、辅酶Q9和胆固醇）的特定分布使我们能够在小鼠腿部切片上区分出三个不同区域：一个已被破坏，另一个正在退化（氧化应激和磷酸肌醇循环失调），最后一个是稳定的。TOF-SIMS成像显示出能够直接在组织切片上定位大量反映细胞代谢状态的脂质化合物的能力。
