Sjövall Peter, Rossmeisl Martin, Hanrieder Jörg, Kuda Ondrej, Kopecky Jan, Bryhn Morten
Chemistry, Materials and Surfaces, SP Technical Research Institute of Sweden, 50115, Borås, Sweden,
Anal Bioanal Chem. 2015 Jul;407(17):5101-11. doi: 10.1007/s00216-015-8515-7. Epub 2015 Feb 19.
Dietary intake of omega-3 fatty acids is associated with considerable health benefits, including the prevention of metabolic disorders such as cardiovascular disease and type 2 diabetes. Furthermore, incorporation of the main omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), at the systemic level has been found to be more efficient when these fatty acids are supplied in the form of marine phospholipids compared to triglycerides. In this work, the uptake of omega-3 fatty acids and their incorporation in specific lipids were studied in adipose, skeletal muscle, and liver tissues of mice given high-fat diets with or without omega-3 supplements in the form of phospholipids or triglycerides using time-of-flight secondary ion mass spectrometry (TOF-SIMS). The results demonstrate significant uptake of EPA and DHA, and the incorporation of these fatty acids in specific lipid molecules, in all three tissue types in response to the dietary omega-3 supplements. Moreover, the results indicate reduced concentrations of arachidonic acid (AA) and depletion of lipids containing AA in tissue samples from mice given supplementary omega-3, as compared to the control mice. The effect on the lipid composition, in particular the DHA uptake and AA depletion, was found to be significantly stronger when the omega-3 supplement was supplied in the form of phospholipids, as compared to triglycerides. TOF-SIMS was found to be a useful technique for screening the lipid composition and simultaneously obtaining the spatial distributions of various lipid classes on tissue surfaces.
饮食中摄入omega-3脂肪酸对健康有诸多益处，包括预防心血管疾病和2型糖尿病等代谢紊乱。此外，研究发现，与甘油三酯相比，以海洋磷脂形式提供二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）这两种主要的omega-3脂肪酸时，在全身水平上的吸收效率更高。在这项研究中，利用飞行时间二次离子质谱（TOF-SIMS），研究了给予高脂饮食且添加或不添加磷脂或甘油三酯形式omega-3补充剂的小鼠脂肪组织、骨骼肌组织和肝脏组织中omega-3脂肪酸的摄取及其在特定脂质中的掺入情况。结果表明，在所有三种组织类型中，响应饮食中的omega-3补充剂，EPA和DHA均有显著摄取，且这些脂肪酸掺入了特定的脂质分子中。此外，结果表明，与对照小鼠相比，补充omega-3的小鼠组织样本中花生四烯酸（AA）浓度降低，且含AA的脂质减少。与甘油三酯相比，当以磷脂形式提供omega-3补充剂时，对脂质组成的影响，特别是DHA摄取和AA消耗，明显更强。TOF-SIMS被发现是一种用于筛选脂质组成并同时获取组织表面各种脂质类别的空间分布的有用技术。
