Rebała K, Szczerkowska Z
Department of Forensic Medicine, Medical University of Gdańsk, ul. Marii Skłodowskiej-Curie 3A, 80-210, Gdańsk, Poland.
Int J Legal Med. 2005 Sep;119(5):303-5. doi: 10.1007/s00414-005-0547-7. Epub 2005 Apr 16.
Polymorphism of 18 STR loci specific to the human Y chromosome (DYS19, DYS388, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS426, DYS437, DYS438, DYS439, DYS460, GATA H4.1, DYS385 a/b, and YCAII a/b) was evaluated by means of a multiplex (octadecaplex) PCR reaction and capillary electrophoresis in a Polish population sample of 208 unrelated males. A total of 192 different haplotypes and 183 unique haplotypes were identified. The observed haplotype diversity was 0.998, while discrimination capacity was 92.3%. DYS389 was shown to be the most valuable in discrimination of similar haplotypes, whereas DYS388, DYS393, DYS426, and DYS438 did not affect the discrimination power of the multiplex.
采用多重（十八重）聚合酶链反应和毛细管电泳技术，对208名无亲缘关系的波兰男性群体样本中的18个人类Y染色体特异性短串联重复序列（STR）位点（DYS19、DYS388、DYS389I、DYS389II、DYS390、DYS391、DYS392、DYS393、DYS426、DYS437、DYS438、DYS439、DYS460、GATA H4.1、DYS385 a/b和YCAII a/b）的多态性进行了评估。共鉴定出192种不同单倍型和183种独特单倍型。观察到的单倍型多样性为0.998，而鉴别能力为92.3%。结果表明，DYS389在鉴别相似单倍型方面最具价值，而DYS388、DYS393、DYS426和DYS438对多重检测的鉴别能力没有影响。
