Bouzaine T, Dauphin R D, Thonart Ph, Urdaci M C, Hamdi M
UR-Microbial and Food Process, National Institute of Applied Science and Technology, University of Carthage, Tunis, Tunisia.
Lett Appl Microbiol. 2005;40(5):391-6. doi: 10.1111/j.1472-765X.2005.01684.x.
Selected lactic acid bacteria (LAB) isolated from intestinal tract of chicken have been studied in order to investigate their ability to adhere in vitro to Basement Membrane Matrigel (BMM). A selected strain showing a good adherence in BMM test was used for in vivo colonization assays.
In vitro assessment of adhesion of broiler chicken isolates was performed using BMM assay. Among LAB strains tested, Lactobacillus rhamnosus TB1 showed a good adherence that was comparable to the one of an Escherichia coli EPEC strain used as positive control. For in vivo colonization assays this strain was fluorescently stained with the carboxyfluorescein diacetate succinimidyl ester (cFDA-SE) thus allowing its detection in different layers of intestinal tract after inoculation in broiler chicken. Further, stained L. rhamnosus were found with a highest value in rectum, jejunum and ileum both 3 and 24 h after administration.
BMM assay is a quick method to test in vitro adhesion properties of bacterial strains and cFDA-SE-stained bacteria may be considered as an alternative method to test in vivo adhesion and colonization properties.
Lactobacillus rhamnosus TB1 was therefore showed to be able to adhere strongly in vitro to BMM and in vivo to intestinal epithelial cells of chicken and may be considered as a potential probiotic for chicken.
对从鸡肠道分离出的特定乳酸菌（LAB）进行研究，以考察其体外黏附于基底膜基质胶（BMM）的能力。选用一株在BMM试验中表现出良好黏附性的菌株进行体内定植试验。
采用BMM试验对肉鸡分离株的黏附进行体外评估。在所测试的LAB菌株中，鼠李糖乳杆菌TB1表现出良好的黏附性，与用作阳性对照的大肠杆菌EPEC菌株相当。对于体内定植试验，该菌株用羧基荧光素二乙酸琥珀酰亚胺酯（cFDA-SE）进行荧光染色，从而在接种到肉鸡后能在肠道的不同层中检测到它。此外，在给药后3小时和24小时，在直肠、空肠和回肠中发现经染色的鼠李糖乳杆菌数量最多。
BMM试验是一种快速检测细菌菌株体外黏附特性的方法，cFDA-SE染色细菌可被视为检测体内黏附与定植特性的替代方法。
因此，鼠李糖乳杆菌TB1在体外能强烈黏附于BMM，在体内能黏附于鸡的肠上皮细胞，可被视为鸡的潜在益生菌。
