Ma Y-L, Guo T, Xu Z-R, You P, Ma J-F
College of Life Science, Ningxia University, Yinchuan 750021, China.
Lett Appl Microbiol. 2006 Apr;42(4):369-74. doi: 10.1111/j.1472-765X.2006.01844.x.
The aims of this study were to investigate in vitro the effects of Lactobacillus isolates from a chicken on adhesion of pathogenic Salmonella and Escherichia coli to chicken intestinal mucus obtained from different intestinal regions.
Bacteria were labelled by using methyl-1,2-[(3)H]-thymidine. The bacterial adhesion was assessed by measuring the radioactivity of bacteria adhered to the mucus. The results showed that the abilities of Lactobacillus spp. to bind to the same intestinal mucus were higher than those of pathogenic Salmonella and E. coli. Pretreatment of intestinal mucus with Lactobacillus fermentum and Lactobacillus acidophilus, alone or in combination, reduced the adhesion of the tested pathogens, but the reductive extent of pathogenic adhesion by Lactobacillus spp. in combination was relatively high.
The tested bacteria had different adhesions to mucus glycoproteins isolated from different intestinal regions of chicken. Lactobacillus acidophilus and Lact. fermentum in combination revealed a better ability to inhibit attachments of Salmonella and E. coli to chicken intestinal mucus than Lactobacillus sp. alone.
A mixture of intestinal Lactobacillus spp. from a chicken may play a protective role in excluding pathogenic Salmonella and E. coli from the intestine of chicken.
本研究旨在体外研究从鸡体内分离出的乳酸杆菌对致病性沙门氏菌和大肠杆菌黏附于从不同肠道区域获取的鸡肠道黏液的影响。
使用甲基 - 1,2 - [(3)H] - 胸苷对细菌进行标记。通过测量黏附于黏液的细菌的放射性来评估细菌黏附情况。结果表明，乳酸杆菌属与致病性沙门氏菌和大肠杆菌相比，其黏附于相同肠道黏液的能力更高。单独或联合使用发酵乳杆菌和嗜酸乳杆菌对肠道黏液进行预处理，可降低受试病原体的黏附，但乳酸杆菌属联合使用时对致病性黏附的降低程度相对较高。
受试细菌对从鸡不同肠道区域分离出的黏液糖蛋白具有不同的黏附性。与单独使用乳酸杆菌相比，嗜酸乳杆菌和发酵乳杆菌联合使用显示出更好的抑制沙门氏菌和大肠杆菌黏附于鸡肠道黏液的能力。
鸡体内的肠道乳酸杆菌属混合物可能在将致病性沙门氏菌和大肠杆菌排除在鸡肠道之外方面发挥保护作用。
