枯草芽孢杆菌噬菌体SPP1中一个决定包装DNA量的基因的鉴定。

Identification of a gene in Bacillus subtilis bacteriophage SPP1 determining the amount of packaged DNA.

作者信息

Tavares P, Santos M A, Lurz R, Morelli G, de Lencastre H, Trautner T A

机构信息

Departamento Genética Molecular, Centro de Tecnologia Química e Biológica, Oeiras, Portugal.

出版信息

J Mol Biol. 1992 May 5;225(1):81-92. doi: 10.1016/0022-2836(92)91027-m.

DOI:10.1016/0022-2836(92)91027-m

PMID:1583695

Abstract

The virulent Bacillus subtilis bacteriophage SPP1 encapsidates its DNA by a headful mechanism. Analyzing phage missense mutants, which package less DNA than SPP1 wild-type but show no other affected properties, we have identified a gene whose product is involved in the sizing of phage DNA during maturation. Characterization of this gene and its product provides an experimental access to the poorly understood mechanism of DNA sizing in packaging. The gene (gene 6 or siz) was cloned and sequenced. An open reading frame (ORF) coding for a 57.3 kDa polypeptide was identified. All the single nucleotide substitutions present in different siz mutants affect the net charge of that protein. The gene was further characterized by assignment of several nonsense mutations (sus) to the ORF. Phages carrying the latter type of mutations could be complemented in trans when gene 6 is provided by a plasmid.

摘要

烈性枯草芽孢杆菌噬菌体SPP1通过满头部机制包裹其DNA。通过分析噬菌体错义突变体，这些突变体包装的DNA比SPP1野生型少，但未显示出其他受影响的特性，我们鉴定出一个基因，其产物在成熟过程中参与噬菌体DNA的大小确定。对该基因及其产物的表征为了解包装中DNA大小确定这一 poorly understood机制提供了实验途径。该基因（基因6或siz）被克隆并测序。鉴定出一个编码57.3 kDa多肽的开放阅读框（ORF）。不同siz突变体中存在的所有单核苷酸取代都会影响该蛋白质的净电荷。通过将几个无义突变（sus）定位到ORF对该基因进行了进一步表征。当质粒提供基因6时，携带后一种类型突变的噬菌体可以通过反式互补。