Mendez Susana, Zhan Bin, Goud Gaddam, Ghosh Kashinath, Dobardzic Azra, Wu Wenhui, Liu Sen, Deumic Vehid, Dobardzic Reshad, Liu Yueyuan, Bethony Jeff, Hotez Peter J
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, Rose Hall 736, 2300 Eye Street, NW, Washington, DC 20037, USA.
Vaccine. 2005 May 2;23(24):3123-30. doi: 10.1016/j.vaccine.2004.12.022.
Syrian Golden hamsters were vaccinated with the recombinant fusion proteins Ay-ASP-2 and Ay-MTP-1 from the infective larvae of the hookworm Ancylostoma ceylanicum. Vaccines comprised each antigen alone or the combination of the two proteins. All vaccinated group developed high antibody titers (>1:40,000); coadministration of a second antigen did not significantly affect the magnitude of the antibody response. Following challenge, hamsters vaccinated with each single antigen exhibited reductions in worm burden (32% and 28% to Ay-ASP-2 and Ay-MTP-1, respectively) and fecal egg counts (56% and 43%, respectively). A vaccine cocktail, containing both antigens further reduced worm burden (36%) and fecal egg counts (59%) (p<0.001). Moreover, vaccination with the antigen cocktail significantly improved hemoglobin values (p=0.01) and body weights (p=0.001) compared to what achieved with either each antigen or adjuvant alone. Taken together, these data suggest that combination of two or more antigens may present an effective vaccine development strategy to improve protection and/or disease symptoms in affected individuals.
用来自锡兰钩虫感染性幼虫的重组融合蛋白Ay-ASP-2和Ay-MTP-1对叙利亚金仓鼠进行免疫接种。疫苗包括单独的每种抗原或两种蛋白的组合。所有接种疫苗的组均产生了高抗体滴度（>1:40,000）；同时给予第二种抗原并未显著影响抗体反应的强度。攻虫后，接种每种单一抗原的仓鼠蠕虫负荷降低（分别为Ay-ASP-2和Ay-MTP-1的32%和28%），粪便虫卵计数降低（分别为56%和43%）。含有两种抗原的疫苗混合物进一步降低了蠕虫负荷（36%）和粪便虫卵计数（59%）（p<0.001）。此外，与单独使用每种抗原或佐剂相比，接种抗原混合物显著改善了血红蛋白值（p=0.01）和体重（p=0.001）。综上所述，这些数据表明，两种或更多种抗原的组合可能是一种有效的疫苗开发策略，可改善受影响个体的保护和/或疾病症状。
