Chang Lin-Chau, Lin Chia-Yu, Kuo Min-Wen, Gau Churn-Shiouh
School of Pharmacy, College of Medicine, National Taiwan University, No. 1, Sect. 1, Jen-Ai Rd., Taipei, Taiwan.
J Colloid Interface Sci. 2005 May 15;285(2):640-52. doi: 10.1016/j.jcis.2004.11.011.
Pluronics are triblock copolymers which are extensively applied excipients shown to interact with cell membranes. The aim of our study was to apply monolayer techniques and epifluorescence microscopy to investigate the interaction behavior between selected Pluronics and phospholipid monolayers which serve as a model of cell membranes. The results showed that Pluronic L61 with hydrophobic proportions much larger than those of F68 demonstrated condensed film-like surface behavior while F68 exhibited more expanded behavior. The increments of surface pressure and the changes of image were more obvious in adding Pluronic L61 than F68 to the subphase of dipalmitoylphosphatidylcholine (DPPC) monolayers, which indicated that the interaction may be related to van der Waals forces and hydrophobic interaction. Pluronics selected with higher hydrophobicities demonstrated larger surface activities and penetration abilities while being added to the subphase of DPPC and dimyristoylphosphatidylcholine (DMPC) monolayers. Pluronic P85 and F68 were found to be squeezed to subphase at higher surface pressures, which may be attributed to their relatively higher hydrophilicities.
普朗尼克是三嵌段共聚物，是广泛应用的辅料，已证明其能与细胞膜相互作用。我们研究的目的是应用单层技术和落射荧光显微镜来研究选定的普朗尼克与作为细胞膜模型的磷脂单层之间的相互作用行为。结果表明，疏水比例远大于F68的普朗尼克L61表现出凝聚膜状的表面行为，而F68表现出更伸展的行为。在二棕榈酰磷脂酰胆碱（DPPC）单层的亚相中添加普朗尼克L61比添加F68时，表面压力的增加和图像的变化更明显，这表明这种相互作用可能与范德华力和疏水相互作用有关。在添加到DPPC和二肉豆蔻酰磷脂酰胆碱（DMPC）单层的亚相中时，选择的具有较高疏水性的普朗尼克表现出更大的表面活性和渗透能力。发现普朗尼克P85和F68在较高表面压力下被挤压到亚相中，这可能归因于它们相对较高的亲水性。
