Yordanov Georgi, Minkov Ivan, Balashev Konstantin
Department of Inorganic Chemistry, Faculty of Chemistry and Pharmacy, Sofia University St. Kliment Ohridski, 1 "James Bourchier" Blvd., 1164 Sofia, Bulgaria.
Department of Chemistry, Biochemistry, Physiology, and Pathophysiology, Faculty of Medicine, Sofia University, 1407 Sofia, Bulgaria.
Membranes (Basel). 2024 Dec 2;14(12):254. doi: 10.3390/membranes14120254.
Poly(butyl cyanoacrylate) (PBCA) nanoparticles have numerous applications, including drug and gene delivery, molecular imaging, and cancer therapy. To uncover the molecular mechanisms underlying their interactions with cell membranes, we utilized a Langmuir monolayer as a model membrane system. This approach enabled us to investigate the processes of penetration and reorganization of PBCA nanoparticles when deposited in a phospholipid monolayer subphase. Atomic force microscopy (AFM) was employed to visualize Langmuir-Blodgett (LB) films of these nanoparticles. Additionally, we examined the state of a monolayer of Pluronic F68, a stabilizer of PBCA nanoparticles in suspension, by measuring the changes in relative surface area and surface potential over time in the barostatic regime following PBCA suspension spreading. Based on these findings, we propose a molecular mechanism for nanoparticle reorganization at the air-water interface.
聚氰基丙烯酸丁酯（PBCA）纳米颗粒有众多应用，包括药物和基因递送、分子成像以及癌症治疗。为了揭示其与细胞膜相互作用的分子机制，我们将朗缪尔单分子层用作模型膜系统。这种方法使我们能够研究PBCA纳米颗粒沉积在磷脂单分子层亚相中时的渗透和重组过程。采用原子力显微镜（AFM）来观察这些纳米颗粒的朗缪尔-布洛杰特（LB）膜。此外，我们通过测量PBCA悬浮液铺展后在恒压状态下相对表面积和表面电位随时间的变化，研究了悬浮液中PBCA纳米颗粒的稳定剂普朗尼克F68单分子层的状态。基于这些发现，我们提出了纳米颗粒在气-水界面重组的分子机制。
