Yan Yuan, Liu Shi-Ting, Xu Zhong-Yuan, Luo Qi-Zhi, Tan Ya-Fei, Chen Zhi-Liang
Department of Pharmaceutical Science, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2005 Apr;25(4):447-9, 453.
To identify the active components of compound dandelion enema, a preparation from 7 traditional Chinese herbal drugs for treatment of gynecological diseases.
Three-dimensional high-performance liquid chromatography (3D-HPLC) was employed to separate the ethyl acetate extract of compound dandelion Enema, and HPLC combined with mass spectrum (MS) analysis used for chromatographic fingerprinting.
By comparing the ionic fragments of MS and retention time of each peak, the main active components in compound dandelion enema were determined, including caffeic acid, ferulic acid and protocatechualdehyde.
HPLC coupled with mass spectroscopy can be used for qualitative analysis of compound dandelion enema.
鉴定复方蒲公英灌肠剂的活性成分，该制剂由7味治疗妇科疾病的传统中药制成。
采用三维高效液相色谱法（3D-HPLC）分离复方蒲公英灌肠剂的乙酸乙酯提取物，并采用HPLC结合质谱（MS）分析进行色谱指纹图谱分析。
通过比较MS的离子碎片和各峰的保留时间，确定了复方蒲公英灌肠剂中的主要活性成分，包括咖啡酸、阿魏酸和原儿茶醛。
HPLC联用质谱可用于复方蒲公英灌肠剂的定性分析。
