高速逆流色谱法从蒲公英中制备分离和纯化三种黄酮苷

Preparative isolation and purification of three flavonoid glycosides from Taraxacum mongolicum by high-speed counter-current chromatography.

作者信息

Shi Shuyun, Zhang Yuping, Zhao Yu, Huang Kelong

机构信息

School of Chemistry and Chemical Engineering, Central South University, Changsha, China.

出版信息

J Sep Sci. 2008 Mar;31(4):683-8. doi: 10.1002/jssc.200700409.

DOI:10.1002/jssc.200700409

PMID:18240133

Abstract

A preparative high-speed counter-current chromatography (HSCCC) was successively applied to purify three flavonoid glycosides from the aerial part of Taraxacum mongolicum, a traditional Chinese medicine. Subsequent UV, MS, and NMR analyses have led to the characterization of three flavonoid glycosides including two new compounds isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-L-arabinopyranoside and isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-D-glucopyranoside, and a known compound, isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-D-xyloypyranoside, which were first isolated from T. mongolicum. The two-phase solvent system composed of ethyl acetate/n-butanol/water (2:1:3, v/v/v) was performed in HSCCC. Consequently, a total of 25.7 mg isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-L-arabinopyranoside, 19.1 mg isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-D-glucopyranoside, and 10.6 mg isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-D-xyloypyranoside were obtained with purity of 98.7, 98.3, and 99.1%, respectively, as determined by HPLC from 500 mg enriched extract after cleaning-up by polyamide resin.

摘要

采用制备型高速逆流色谱法（HSCCC）从中药蒲公英地上部分中依次纯化出三种黄酮苷。随后通过紫外、质谱和核磁共振分析鉴定出三种黄酮苷，其中包括两种新化合物异荭草素 -7-O-β-D-吡喃葡萄糖基-2'-O-α-L-吡喃阿拉伯糖苷和异荭草素 -7-O-β-D-吡喃葡萄糖基-2'-O-α-D-吡喃葡萄糖苷，以及一种已知化合物异荭草素 -7-O-β-D-吡喃葡萄糖基-2'-O-α-D-吡喃木糖苷，它们首次从蒲公英中分离得到。HSCCC采用乙酸乙酯/正丁醇/水（2:1:3，v/v/v）组成的两相溶剂体系。经聚酰胺树脂净化后的500 mg富集提取物，通过高效液相色谱法测定，最终分别获得了25.7 mg异荭草素 -7-O-β-D-吡喃葡萄糖基-2'-O-α-L-吡喃阿拉伯糖苷、19.1 mg异荭草素 -7-O-β-D-吡喃葡萄糖基-2'-O-α-D-吡喃葡萄糖苷和10.6 mg异荭草素 -7-O-β-D-吡喃葡萄糖基-2'-O-α-D-吡喃木糖苷，纯度分别为98.7%、98.3%和99.1%。