Ticho U, Lahav M, Berkowitz S, Yoffe P
Br J Ophthalmol. 1979 Sep;63(9):646-50. doi: 10.1136/bjo.63.9.646.
Rabbit eyes with steroid-induced ocular hypertension were investigated in order to evaluate the histochemical abnormalities in the chamber angle region. The right eye of 14 rabbits was treated by dexamethasone 1% 3 times daily for 3 to 5 weeks. The eyes were stained by haematoxylin-eosin, periodic acid Schiff, fibrin, colloidal iron, and alcian blue with and without hyaluronidase. All treated eyes developed elevated intraocular pressure up to 4 weeks after treatment. These globes showed alcian-blue-positive hyaluronidase-sensitive staining in the amorphous material adjacent to Schlemm's canal and in the cytoplasmic granules of trabecular endothelial cells. There was no increase in incorporation of radioactive thymidine into nuclei of endothelial cells as seen by autoradiography. These results provide further support for the idea that there is abnormal accumulation of acid mucopolysaccharides in the chamber angle in steroid-induced ocular hypertension.
为评估房角区域的组织化学异常，对患有类固醇性高眼压的兔眼进行了研究。14只兔子的右眼每日用1%地塞米松治疗3次，持续3至5周。用苏木精-伊红、过碘酸希夫、纤维蛋白、胶体铁以及有无透明质酸酶的阿尔辛蓝对眼睛进行染色。所有治疗眼在治疗后长达4周时眼压升高。这些眼球在施莱姆管附近的无定形物质以及小梁内皮细胞的细胞质颗粒中显示出对透明质酸酶敏感的阿尔辛蓝阳性染色。通过放射自显影未观察到内皮细胞核中放射性胸苷掺入增加。这些结果为类固醇性高眼压时房角酸性黏多糖异常蓄积的观点提供了进一步支持。
