Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China.
Acta Ophthalmol. 2012 Sep;90(6):559-63. doi: 10.1111/j.1755-3768.2010.02016.x. Epub 2010 Nov 2.
To investigate the ocular hypertensive response to topical dexamethasone (DEX), rimexolone (RIM), loteprednol etabonate (LOT) and fluorometholone (FML) in rabbits of different ages.
Seventy-five rabbits of three age groups (7 weeks, 6 months and 1-year old) received topical administration of 0.1% DEX, 1% RIM, 0.5% LOT, 0.1% FML or balanced salt solution four times daily for 1 month. Intraocular pressure (IOP) was monitored at regular time intervals. After a month, eyes were harvested for histological study with haematoxylin and eosin (H&E), periodic acid Schiff and Masson trichrome staining. Trabecular meshwork changes were graded by masked ocular pathologists.
Topical DEX caused the greatest increase in IOP, followed by RIM and FML. LOT caused the least IOP increase. Similar pattern of IOP response to the four corticosteroids was observed in the three studied age groups. Young rabbits (7 week) were the most responsive to corticosteroids among the age groups. Extracellular matrix thickening in the trabecular meshwork region and loss of trabecular meshwork cells were observed after DEX, FML or RIM treatments.
Young rabbits are more susceptible to steroid induced increase in IOP, even for milder steroids such as fluorometholone and rimexolone.
研究不同年龄兔局部应用地塞米松（DEX）、利美索龙（RIM）、洛度沙胺（LOT）和氟米龙（FML）的眼压升高反应。
75 只兔子分为 3 组（7 周龄、6 月龄和 1 岁龄），每日 4 次给予 0.1% DEX、1% RIM、0.5% LOT、0.1% FML 或平衡盐溶液滴眼，持续 1 个月。定期监测眼压。1 个月后，对眼球进行苏木精-伊红（H&E）、过碘酸希夫（periodic acid Schiff，PAS）和 Masson 三色染色的组织学研究。用盲法眼科病理学家对小梁网变化进行分级。
局部 DEX 引起的眼压升高最大，其次是 RIM 和 FML。LOT 引起的眼压升高最小。在研究的 3 个年龄组中，四种皮质类固醇的眼压反应模式相似。在年龄组中，7 周龄的幼兔对皮质类固醇的反应最敏感。在 DEX、FML 或 RIM 治疗后，观察到小梁网区域细胞外基质增厚和小梁网细胞丢失。
幼兔对类固醇引起的眼压升高更敏感，即使是氟米龙和利美索龙等轻度类固醇也是如此。
