Efrati Ari, Eyal Yoram, Paran Ilan
Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
Genome. 2005 Apr;48(2):347-51. doi: 10.1139/g04-119.
The chlorophyll retainer (cl) mutation causes inhibition of chlorophyll degradation during pepper fruit ripening and is controlled by a single recessive gene. The retention of chlorophyll in mature red or yellow fruits produces brown- or green-colored ripe fruits, respectively. We mapped CL on chromosome 1 of pepper corresponding to chromosome 8 in tomato in which a homologous mutation, green flesh, was previously assigned. To test whether known structural genes from the chlorophyll catabolism pathway could correspond to CL, we mapped tomato expressed sequence tag clones corresponding to three loci of CHLOROPHYLLASE and one locus of PHEOPHORBIDE A OXYGENASE in the tomato introgression lines population. The three CHLOROPHYLLASE loci mapped to chromosomes 6, 9, and 12, while PHEOPHORBIDE A OXYGENASE mapped to chromosome 11, indicating that CL may correspond to an as yet unavailable gene from the chlorophyll catabolism pathway or to a regulator of the pathway.
叶绿素保留（cl）突变导致辣椒果实成熟过程中叶绿素降解受到抑制，且该性状由单个隐性基因控制。成熟的红色或黄色果实中叶绿素的保留分别产生褐色或绿色的成熟果实。我们将CL定位在辣椒的1号染色体上，该染色体对应于番茄的8号染色体，之前在番茄中已定位了一个同源突变——绿果肉。为了测试叶绿素分解代谢途径中已知的结构基因是否与CL相对应，我们在番茄渐渗系群体中定位了与叶绿素酶的三个位点和脱镁叶绿酸a加氧酶的一个位点相对应的番茄表达序列标签克隆。叶绿素酶的三个位点分别定位到6号、9号和12号染色体上，而脱镁叶绿酸a加氧酶定位到11号染色体上，这表明CL可能对应于叶绿素分解代谢途径中一个尚未找到的基因，或者对应于该途径的一个调节因子。
